Laurila P, Stenman S
Department of Pathology, University of Helsinki, Finland.
Int J Dev Biol. 1989 Mar;33(1):63-70.
In the present paper results from our studies on the expression and regulation of the differentiated normal and transformed epithelial and fibroblastic phenotypes are reviewed. The expression of the extracellular matrix- and basement membrane-associated proteins, fibronectin and laminin, and different intermediate filament proteins were studied in different fused cells. Heterokaryons and cytoplasmic hybrids (cybrids) were formed by fusing normal or malignant epithelial cells with normal fibroblasts or malignant glial cells. No differences were observed in the expression of these phenotypic markers between unfused parental cells and the corresponding homokaryons. Thus, the fusion process itself does not cause changes in the expression of these phenotypic markers. In heterokaryons formed after fusion of normal or malignant epithelial cells with normal fibroblasts or malignant glial cells, two or even three different types of intermediate filaments could be co-expressed. Thus, no suppression of the expression of the various intermediate filaments is caused by the non-homologous parental cell. Expression of the extracellular matrix proteins, fibronectin and laminin, on the other hand, could be extinguished by cells and cytoplasts not expressing fibronectin matrix, such as transformed fibroblasts and HeLa cells. These results support the role of transacting regulatory factors controlling the expression of the different extracellular matrix proteins. Alternatively, the results can be explained by changes in the distribution and concentration of the extracellular matrix receptor proteins in the fused cells.
本文综述了我们关于分化的正常及转化的上皮和成纤维细胞表型的表达与调控的研究结果。在不同的融合细胞中研究了细胞外基质和基底膜相关蛋白、纤连蛋白和层粘连蛋白以及不同中间丝蛋白的表达。通过将正常或恶性上皮细胞与正常成纤维细胞或恶性神经胶质细胞融合形成异核体和细胞质杂种（胞质杂种）。在未融合的亲本细胞和相应的同核体之间，未观察到这些表型标志物表达的差异。因此，融合过程本身不会导致这些表型标志物表达的变化。在正常或恶性上皮细胞与正常成纤维细胞或恶性神经胶质细胞融合后形成的异核体中，两种甚至三种不同类型的中间丝可以共表达。因此，非同源亲本细胞不会导致各种中间丝表达的抑制。另一方面，细胞外基质蛋白纤连蛋白和层粘连蛋白的表达可以被不表达纤连蛋白基质的细胞和胞质体（如转化的成纤维细胞和HeLa细胞）所消除。这些结果支持了反式作用调节因子在控制不同细胞外基质蛋白表达中的作用。或者，这些结果可以用融合细胞中细胞外基质受体蛋白的分布和浓度变化来解释。
