在日本三角涡虫中评估通过多巴胺能传递介导的甲基苯丙胺引起的行为过度活跃的药理学作用。

Pharmacological assessment of methamphetamine-induced behavioral hyperactivity mediated by dopaminergic transmission in planarian Dugesia japonica.

机构信息

Department of Clinical and Translational Physiology, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan.

Department of Clinical and Translational Physiology, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan; Department of Biophysics, Graduate School of Science, Kyoto University, Oiwake, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan.

出版信息

Biochem Biophys Res Commun. 2014 Jul 11;449(4):412-8. doi: 10.1016/j.bbrc.2014.05.059. Epub 2014 May 22.

DOI:10.1016/j.bbrc.2014.05.059

PMID:24858686

Abstract

The freshwater planarian Dugesia japonica has a simple central nervous system (CNS) and can regenerate complete organs, even a functional brain. Recent studies demonstrated that there is a great variety of neuronal-related genes, specifically expressed in several domains of the planarian brain. We identified a planarian dat gene, named it D. japonica dopamine transporter (Djdat), and analyzed its expression and function. Both in situ hybridization and immunofluorescence revealed that localization of Djdat mRNA and protein was the same as that of D. japonica tyrosine hydroxylase (DjTH). Although, dopamine (DA) content in Djdat(RNAi) planarians was not altered, Djdat(RNAi) planarians showed increased spontaneous locomotion. The hyperactivity in the Djdat(RNAi) planarians was significantly suppressed by SCH23390 or sulpiride pretreatment, which are D1 or D2 receptor antagonists, respectively. These results suggest that planarians have a Djdat ortholog and the ability to regulate dopaminergic neurotransmission and association with spontaneous locomotion.

摘要

日本三角涡虫具有简单的中枢神经系统（CNS），可以再生完整的器官，甚至是功能性大脑。最近的研究表明，存在大量与神经元相关的基因，这些基因特异性地在涡虫大脑的几个区域表达。我们鉴定了一种涡虫 dat 基因，将其命名为日本三角涡虫多巴胺转运体（Djdat），并分析了其表达和功能。原位杂交和免疫荧光均显示，Djdat mRNA 和蛋白质的定位与日本三角涡虫酪氨酸羟化酶（DjTH）相同。尽管 Djdat（RNAi）涡虫中的多巴胺（DA）含量没有改变，但 Djdat（RNAi）涡虫表现出自发性运动增加。SCH23390 或 sulpiride 预处理可显著抑制 Djdat（RNAi）涡虫的过度活跃，SCH23390 和 sulpiride 分别是 D1 或 D2 受体拮抗剂。这些结果表明，涡虫具有 Djdat 直系同源物，并且能够调节多巴胺能神经传递，并与自发性运动有关。