Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.
Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.
Bioresour Technol. 2014 Jul;164:292-8. doi: 10.1016/j.biortech.2014.04.075. Epub 2014 May 5.
To improve ethanol productivity with few inhibitors generated, a novel process of combined alkaline peroxide (AP) pretreatment and semi-simultaneous saccharification and fermentation (SSSF) was developed in this work. Pretreatment with 10% (g H2O2/g wood) H2O2 at 160°C for 2h followed by SSSF was found to be the optimal combination with remarkably increased ethanol yield. The proposed process resulted in 63.1% of ethanol yield, which was about five times more than that of the untreated sample that was processed using conventional simultaneous saccharification and fermentation (SSF). The efficient conversion was ascribed to the high delignification efficiency (64.9%) of AP pretreatment, which led to incompact structure and generation of fewer inhibitors during SSSF (c. 6g/L of lactic acid) than SSF (c. 10 g/L of lactic acid). This combined approach was proved to be an effective method for the promotion of the bioconversion of lignocellulosic materials.
为了提高乙醇的产量，同时减少抑制剂的生成，本工作开发了一种新型的碱过氧化氢（AP）预处理与半同步糖化发酵（SSSF）相结合的工艺。结果发现，160°C 下用 10%（g H2O2/g 木材）H2O2 预处理 2h 后再进行 SSSF 是最佳组合，可显著提高乙醇产量。与未经处理的样品相比，采用常规同步糖化发酵（SSF）处理的未经处理的样品的乙醇得率约提高了 5 倍，达到 63.1%。这种高效转化归因于 AP 预处理的高脱木质素效率（64.9%），这导致在 SSSF 过程中生成的结构不紧密，抑制剂生成较少（约 6g/L 乳酸），而 SSF 中（约 10g/L 乳酸）则较多。该组合方法被证明是促进木质纤维素材料生物转化的有效方法。
