Labat-Robert J, Robert L
Hotel-Dieu Hospital, Paris V University, Paris, France.
Interdiscip Top Gerontol. 2014;39:108-41. doi: 10.1159/000358902. Epub 2014 May 13.
In this chapter, we describe in detail the age-dependent modifications of connective tissues, separately for their cellular and extracellular compartments. Cell aging was studied by the in vitro method established by Hayflick as well as by ex vivo explant cultures, and results with both methods are discussed. Follows then the description of age changes of macromolecular components of extracellular matrix as well as the decline with age of receptor-mediated cell-matrix interactions. These interactions mediated by several types of receptors, as integrins, the elastin receptor and others, play a crucial role for the definition and regulation of the differentiated cell phenotype. Age-related modifications of both matrix components and receptors are discussed in order to explain the mechanisms of the age-dependent modulations of cell-matrix interactions. Finally, we discuss the relations between age changes of matrix components and the onset of age-related diseases, especially cardiovascular pathologies mostly involved in age-dependence of functions and limitation of longevity.
在本章中，我们将分别针对结缔组织的细胞和细胞外区室，详细描述其随年龄的变化。我们通过Hayflick建立的体外方法以及离体组织培养来研究细胞衰老，并对两种方法的结果进行了讨论。接下来描述细胞外基质大分子成分的年龄变化以及受体介导的细胞与基质相互作用随年龄的下降。这些由多种类型的受体介导的相互作用，如整合素、弹性蛋白受体等，对于分化细胞表型的定义和调节起着至关重要的作用。我们讨论了基质成分和受体与年龄相关的变化，以解释细胞与基质相互作用随年龄调节的机制。最后，我们讨论了基质成分的年龄变化与年龄相关疾病的发生之间的关系，特别是主要涉及功能年龄依赖性和寿命限制的心血管疾病。
