Naughton K M, O'Hara T D, Appleton B, Gardner M G
Sciences, Museum Victoria, GPO Box 666, Melbourne, Victoria 3000, Australia; Department of Genetics, Faculty of Science, University of Melbourne, Parkville, Victoria 3010, Australia.
Sciences, Museum Victoria, GPO Box 666, Melbourne, Victoria 3000, Australia.
Mol Phylogenet Evol. 2014 Sep;78:160-71. doi: 10.1016/j.ympev.2014.05.006. Epub 2014 May 24.
The marine species of the southern coast of Australia have not been well studied with regard to molecular connectivity. Cryptic species are expected to be prevalent on this coastline. Here, we investigate the crinoid genus Cenolia (Echinodermata: Crinoidea: Comasteridae) using molecular methods to elucidate cryptic species and phylogenetic relationships. The genus Cenolia dominates the southern Australian crinoid fauna in shallow waters. Few studies have examined crinoids for cryptic species at a molecular level and these have been predominantly based on mitochondrial data. We employ the nuclear markers 28S rRNA and ITS-2 in addition to the mitochondrial COI. Six divergent mitochondrial clades were identified. Gene flow between confirmed clades was subsequently examined by the use of six novel microsatellite markers, showing that sympatric taxa with low mtDNA divergences (1.7% K2P) were not interbreeding in the wild. The type specimens of Cenolia benhami and C. spanoschistum were examined, as well as all six divergent clades. Morphological characters dividing taxa were refined. Due to comb pinnule morphology, the New Zealand species benhami was determined to belong to the genus Oxycomanthus (nov. comb.). Three new species of Cenolia (including the Australian "benhami") require description.
就分子连通性而言，澳大利亚南部海岸的海洋物种尚未得到充分研究。预计在这条海岸线上隐秘物种很普遍。在这里，我们使用分子方法研究海百合属（棘皮动物门：海百合纲：Comasteridae科），以阐明隐秘物种和系统发育关系。Cenolia属在澳大利亚南部浅海海百合动物群中占主导地位。很少有研究在分子水平上检查海百合的隐秘物种，而且这些研究主要基于线粒体数据。除了线粒体细胞色素氧化酶亚基I（COI）外，我们还使用了核标记28S核糖体RNA（rRNA）和内转录间隔区2（ITS-2）。鉴定出六个不同的线粒体分支。随后通过使用六个新的微卫星标记检查了已确认分支之间的基因流，结果表明线粒体DNA分歧较低（K2P为1.7%）的同域分类群在野外没有杂交。检查了Cenolia benhami和C. spanoschistum的模式标本以及所有六个不同的分支。细化了区分分类群的形态特征。由于栉羽枝形态，新西兰物种benhami被确定属于Oxycomanthus属（新组合）。需要描述三个新的Cenolia物种（包括澳大利亚的“benhami”）。
