Kostro K
Klinika Chorób Zakaźnych Zwierzat AR w Lublinie.
Pol Arch Weter. 1989;29(3-4):5-23.
In the studies it was revealed that the content of the medium determines growth and appearance of T. equinum colony. On solid Sabouraud media two types of colonies were observed, however, colonies with characteristic radial folds and grooves in the paracentral zone and umbilical elevation in the centre dominated. As typical features of T. equinum one can accept dark red pigmentation of the reverse side of colony in the culture on solid Sabouraud medium with glucose and yellow and orange pigmentation on the same medium with no glucose. T. equinum strains which have lost the ability of creating characteristic dark red pigment regain that ability after a few passages on media with soil or after just one passage on laboratory animals. None of the studied T. equinum strains grew on medium with casamino acids free of vitamins, while they revealed rapid growth on the same medium with nicotinic acid. T. equinum strains which were used for the studies rapidly adopted to liquid Sabouraud medium and this made it possible to obtain sufficient quantity of mycelium for studies on ++pathogenicity and antigenic structure. On the basis of observations of growth on solid media and mass of mycelium gained on liquid media it was decided that the optimal temperature of incubation of T. equinum is 26-28 degrees C.
研究表明，培养基的成分决定了马疫锥虫菌落的生长和外观。在固体沙氏培养基上观察到两种类型的菌落，然而，在中央旁区域具有特征性放射状褶皱和沟槽且中央有脐状隆起的菌落占主导。作为马疫锥虫的典型特征，可以认为在含葡萄糖的固体沙氏培养基上培养时菌落背面呈暗红色色素沉着，而在不含葡萄糖的相同培养基上呈黄色和橙色色素沉着。失去产生特征性暗红色色素能力的马疫锥虫菌株，在含土壤的培养基上传代几次后或仅在实验动物上传代一次后就恢复了该能力。所研究的马疫锥虫菌株在不含维生素的酪蛋白氨基酸培养基上均不生长，而在添加烟酰胺的相同培养基上生长迅速。用于研究的马疫锥虫菌株能迅速适应液体沙氏培养基，这使得能够获得足够数量的菌丝体用于致病性和抗原结构研究。根据在固体培养基上的生长观察以及在液体培养基上获得的菌丝体量，确定马疫锥虫的最佳培养温度为26 - 28摄氏度。
