Sinha Deepak K, Smith C Michael
Department of Entomology, Kansas State University, Manhattan, KS 66506-4004, USA.
Sci Rep. 2014 May 27;4:5059. doi: 10.1038/srep05059.
The Russian wheat aphid (RWA), Diuraphis noxia Kurdjumov, is a major global pest of wheat and barley production that causes enormous economic damage. Few studies have been conducted to explore and decipher the molecular basis of RWA strategies to evade plant defense mechanisms. Gene expression studies of RWA in response to wheat genotypes carrying different RWA resistance genes have been initiated in our group; however, a secure and accurate understanding of RWA gene expression is dependent on identification of suitable reference genes. This study analyzed expression profiles of five potential reference genes selected and sequenced during RNA sequencing experiments. The expression of genes coding for actin and ribosomal protein L27 was comparatively less variable in RWA fed on different wheat hosts. Results of geNorm, NormFinder, and BestKeeper expression analyses support the use of actin and ribosomal protein L27 in RT-qPCR studies of RWA gene expression in studies involving RWA-wheat interactions.
俄罗斯小麦蚜虫（RWA），即麦二叉蚜（Diuraphis noxia Kurdjumov），是全球小麦和大麦生产中的主要害虫，会造成巨大的经济损失。很少有研究去探索和解读RWA规避植物防御机制的分子基础。我们团队已开展了RWA对携带不同RWA抗性基因的小麦基因型的基因表达研究；然而，对RWA基因表达的可靠且准确的理解依赖于合适的内参基因的鉴定。本研究分析了在RNA测序实验中选择并测序的五个潜在内参基因的表达谱。在取食不同小麦寄主的RWA中，编码肌动蛋白和核糖体蛋白L27的基因表达变化相对较小。geNorm、NormFinder和BestKeeper表达分析结果支持在涉及RWA与小麦相互作用的研究中，将肌动蛋白和核糖体蛋白L27用于RWA基因表达的RT-qPCR研究。
