Lau Chun Yat, Roslan Zulhanif, Cheong Brandon Huey-Ping, Chua Wei Seong, Liew Oi Wah, Ng Tuck Wah
Laboratory for Optics and Applied Mechanics, Monash University, Clayton VIC3800, Australia.
Cardiovascular Research Institute, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Centre for Translational Medicine, 14 Medical Drive, Singapore 117599, Singapore.
J Colloid Interface Sci. 2014 Jul 15;426:56-63. doi: 10.1016/j.jcis.2014.03.048. Epub 2014 Apr 2.
Transparency microplates enable biochemical analysis in resource-limited laboratories. During the process of transfer, the analytes tittered into the wells may undergo spillage from one well to another due to lateral impact. Sidelong impact tests conducted found the absence of non-linear effects (e.g., viscoelastic behavior) but high energy loss. Finite element simulations conducted showed that the rectangular plate holding the transparencies could undergo z-axis deflections when a normal component of the force was present despite constraints being used. High speed camera sequences confirmed this and also showed the asymmetrical z-axis deflection to cause the contact line closer to impact to displace first when the advancing condition was exceeded. Capillary waves were found to travel toward the contact line at the opposite end, where if the advancing contact angle condition was exceeded, also resulted in spreading. The presence of surface scribing was found to limit contact line movement better. With water drops dispensed on scribed transparencies, immunity from momentum change of up to 9.07 kgm/s on impact was possible for volumes of 40 μL. In the case of glycerol drops immunity from momentum change of up to 9.07 kgm/s on impact extended to volumes of 90 μL. The improved immunity of glycerol was attributed to its heightened dampening characteristics and its higher attenuation of capillary waves. Overall, scribed transparency microplates were able to better withstand spillage from accidental impact. Accidental impact was also found not to cause any detrimental effects on the fluorescence properties of enhanced green fluorescent protein samples tested.
透明微孔板能够在资源有限的实验室中进行生化分析。在转移过程中，滴定到孔中的分析物可能会因侧向冲击而从一个孔溢出到另一个孔。进行的侧向冲击测试发现不存在非线性效应（例如粘弹性行为）但能量损失较高。进行的有限元模拟表明，尽管使用了约束，但当存在力的法向分量时，装有透明片的矩形板可能会发生z轴偏转。高速摄像机序列证实了这一点，并且还显示出不对称的z轴偏转导致在超过前进条件时，靠近冲击点的接触线首先发生位移。发现毛细波向另一端的接触线传播，如果超过前进接触角条件，也会导致扩散。发现表面刻痕的存在能更好地限制接触线的移动。在刻有图案的透明片上滴加水滴时，对于40μL的体积，冲击时动量变化高达9.07 kgm/s仍具有抗性。对于甘油滴，冲击时动量变化高达9.07 kgm/s的抗性可扩展到90μL的体积。甘油抗性的提高归因于其增强的阻尼特性及其对毛细波的更高衰减。总体而言，刻有图案的透明微孔板能够更好地承受意外冲击造成的溢出。还发现意外冲击对所测试的增强型绿色荧光蛋白样品的荧光特性没有任何有害影响。
