Huang Dunyuan, Hao Jiasheng, Zhang Wei, Su Tianjuan, Wang Ying, Xu Xiaofeng
a Institute of Genetic Resources, College of Life Science, Nanjing Normal University , Nanjing , People's Republic of China .
b Laboratory of Molecular Evolution and Biodiversity , College of Life Sciences, Anhui Normal University , Wuhu , People's Republic of China , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(2):806-8. doi: 10.3109/19401736.2014.919452. Epub 2014 May 28.
We sequenced the complete mitochondrial genome of Melanargia asiatica (Lepidoptera: Nymphalidae: Satyrinae). The entire closed circular molecule is 15,142 bp long, containing 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and a AT-rich region. All protein-coding genes (PCGs) initiate with the typical start codons ATN, with the exception of cox1, which uses CGA instead. Nine PCGs use the conventional stop codons (TAA) and the other four genes (cox1, cox2, nad4 and nad5) use a single T as the stop codon. All tRNA genes display typical secondary cloverleaf structures, except for trnS1 (AGN), whose dihydrouridine (DHU) arm is replaced by a simple loop, as observed in all other lepidopterans. The AT-rich region is 319 bp in length and contains some features characteristic of lepidopterans, such as the ATAGA motif followed by a 19-bp poly-T stretch and a microsatellite-like repeat of (TA)6T(TA) preceded by the ATTTA motif.
我们对亚洲矍眼蝶(鳞翅目:蛱蝶科:眼蝶亚科)的完整线粒体基因组进行了测序。整个闭合环状分子长度为15,142 bp,包含13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和一个富含AT的区域。所有蛋白质编码基因(PCGs)均以典型的起始密码子ATN起始,但cox1除外,它使用CGA作为起始密码子。九个PCGs使用传统的终止密码子(TAA),其他四个基因(cox1、cox2、nad4和nad5)使用单个T作为终止密码子。除了trnS1(AGN)外,所有tRNA基因均呈现典型的二级三叶草结构,其双氢尿嘧啶(DHU)臂被一个简单环取代,这在所有其他鳞翅目昆虫中均有观察到。富含AT的区域长度为319 bp,包含一些鳞翅目昆虫特有的特征,如ATAGA基序后接一个19 bp的多聚T序列,以及在ATTTA基序之前的(TA)6T(TA)微卫星样重复序列。