Shen Jinhui, Cong Qian, Grishin Nick V
Department of Biophysics and Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-8816, USA.
Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9050, USA ; Department of Biophysics and Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-8816, USA.
Meta Gene. 2015 Jun 14;5:68-83. doi: 10.1016/j.mgene.2015.05.002. eCollection 2015 Sep.
Due to the intriguing morphology, lifecycle, and diversity of butterflies and moths, Lepidoptera are emerging as model organisms for the study of genetics, evolution and speciation. The progress of these studies relies on decoding Lepidoptera genomes, both nuclear and mitochondrial. Here we describe a protocol to obtain mitogenomes from Next Generation Sequencing reads performed for whole-genome sequencing and report the complete mitogenome of Papilio (Pterourus) glaucus. The circular mitogenome is 15,306 bp in length and rich in A and T. It contains 13 protein-coding genes (PCGs), 22 transfer-RNA-coding genes (tRNA), and 2 ribosomal-RNA-coding genes (rRNA), with a gene order typical for mitogenomes of Lepidoptera. We performed phylogenetic analyses based on PCG and RNA-coding genes or protein sequences using Bayesian Inference and Maximum Likelihood methods. The phylogenetic trees consistently show that among species with available mitogenomes Papilio glaucus is the closest to Papilio (Agehana) maraho from Asia.
由于蝴蝶和蛾类具有迷人的形态、生命周期和多样性，鳞翅目正逐渐成为遗传学、进化和物种形成研究的模式生物。这些研究的进展依赖于对鳞翅目核基因组和线粒体基因组的解码。在此，我们描述了一种从全基因组测序的下一代测序读数中获取线粒体基因组的方法，并报告了北美蓝凤蝶（Papilio (Pterourus) glaucus）的完整线粒体基因组。该环状线粒体基因组长度为15306 bp，富含A和T。它包含13个蛋白质编码基因（PCG）、22个转运RNA编码基因（tRNA）和2个核糖体RNA编码基因（rRNA），其基因顺序是鳞翅目线粒体基因组的典型顺序。我们使用贝叶斯推断和最大似然法，基于PCG、RNA编码基因或蛋白质序列进行了系统发育分析。系统发育树一致表明，在已获得线粒体基因组的物种中，北美蓝凤蝶与来自亚洲的台湾蓝凤蝶（Papilio (Agehana) maraho）最为接近。
