Furuya K
Fukuoka Shika Daigaku Gakkai Zasshi. 1989;16(4):572-99.
The purpose of this study was to evaluate the effect of composite resin monomer on dentinal tubules, odontoblasts and pulp with scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Resin monomers of ethyleneglycol dimethacrylate (EDMA), triethyleneglycol dimethacrylate (Tri-EDMA), urethane dimethacrylate (UDMA), and 2, 2-bis [4- (3-methacryloxy-2-hydroxypropoxy) phenyl] propane (Bis-GMA) were used, and placed on a class V cavity in eighty teeth of adult dogs. Then, these treated animals were sacrificed after 30 to 120 days. The findings obtained were as follows: In the odontoblast body; 1. After 30 days, there were uneven unclear envelopes in all odontoblasts. 2. After 60 and 90 days, there were more invaginations of unclear envelope in Bis-GMA group than those in EDMA, Tri-EDMA and UDMA, and lysosomes and autophagic vacuoles increased in number. 3. In Bis-GMA group after 120 days, there were many degenerative changes of chromatin aggregation and hydropic, and many necrosis cells were seen. Uneven nucleous and lysosomes increased in EDMA, Tri-EDMA and UDMA groups. In the odontoblast process; 4. The odontoblast process disappeared in the dentinal tubule one third of cavity floor side of residual dentin with SEM and TEM after 30 days. However, after 120 days, there were few granular substances and calcified closure was not seen in dentinal tubules. In the region of central one third; 5. Microfilaments and microtubules in the odontoblast process decreased in number, and disarrangement and reticular degeneration of these fibers in all experimental groups after 30 days were seen. After 60 days, shrinkage and reticular degeneration of the odontoblast process were seen. Especially in Bis-GMA group, the odontoblast processes disappeared and the one in other three groups showed reticular degeneration after 90 and 120 days. 6. At the portion of pulp side one third on residual dentin, all odontoblast processes were almost seen normal ultrastructure after 60 days. In the group of Bis-GMA after 90 days, reticular degeneration was seen. Shrinkage of microfilaments and microtubules in EDMA, Tri-EDMA and UDMA groups were slightly seen. In the dentinal tubules after 120 days, reticular degeneration and empty in Bis-GMA group, and disarrangement of microfilaments and microtubules was seen in EDMA, Tri-EDMA and UDMA groups. The above findings indicated that composite resin monomers used in this experiment have effects on the odontoblast respectively. In the clinic, when we restore the cavity with these composite resins, we should intercept the stimulation of residual monomers with perfect dentin lining.
本研究旨在通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）评估复合树脂单体对牙本质小管、成牙本质细胞和牙髓的影响。使用了二甲基丙烯酸乙二醇酯（EDMA）、二甲基丙烯酸三乙二醇酯（Tri-EDMA）、二甲基丙烯酸聚氨酯（UDMA）和2,2-双[4-(3-甲基丙烯酰氧基-2-羟基丙氧基)苯基]丙烷（Bis-GMA）等树脂单体，并将其置于成年犬的80颗牙齿的V类洞上。然后，在30至120天后处死这些处理过的动物。获得的结果如下：在成牙本质细胞体中；1. 30天后，所有成牙本质细胞中出现不均匀、不清晰的包膜。2. 60天和90天后，Bis-GMA组中不清晰包膜的内陷比EDMA、Tri-EDMA和UDMA组更多，溶酶体和自噬泡数量增加。3. 120天后，Bis-GMA组中出现许多染色质聚集和水样变性的退行性变化，可见许多坏死细胞。EDMA、Tri-EDMA和UDMA组中细胞核不均匀，溶酶体增加。在成牙本质细胞突起中；4. 30天后，用SEM和TEM观察到在剩余牙本质洞底侧三分之一的牙本质小管中成牙本质细胞突起消失。然而，120天后，牙本质小管中几乎没有颗粒物质，也未见钙化封闭。在中央三分之一区域；5. 30天后，所有实验组中成牙本质细胞突起中的微丝和微管数量减少，这些纤维出现排列紊乱和网状变性。60天后，观察到成牙本质细胞突起收缩和网状变性。特别是在Bis-GMA组中，90天和120天后成牙本质细胞突起消失，其他三组中的成牙本质细胞突起出现网状变性。6. 在剩余牙本质牙髓侧三分之一部分，60天后所有成牙本质细胞突起的超微结构几乎正常。90天后，Bis-GMA组中出现网状变性。EDMA、Tri-EDMA和UDMA组中微丝和微管略有收缩。120天后，Bis-GMA组牙本质小管中出现网状变性和空虚，EDMA、Tri-EDMA和UDMA组中微丝和微管排列紊乱。上述结果表明，本实验中使用的复合树脂单体分别对成牙本质细胞有影响。在临床上，当我们用这些复合树脂修复洞时，我们应该用完善的牙本质衬层阻断残留单体的刺激。
