采用聚合酶链式反应（PCR）筛选和监测中国饲料中产生玉米赤霉烯酮的镰刀菌属菌种，并采用单克隆抗体检测玉米赤霉烯酮。

Screening and monitoring zearalenone-producing Fusarium species by PCR and zearalenone by monoclonal antibodies in feed from China.

作者信息

Pei Shi-Chun, Zhen Yu-Ping, Gao Jian-Wei, Lee Won-Jong, Zhang Hong-Fu, Ji Cheng, Zhang Xuan-Zhe, Chen Cong

机构信息

a State Key Laboratory of Animal Nutrition , Institute of Animal Science, Chinese Academy of Agricultural Sciences , Beijing , P.R. China.

出版信息

Food Addit Contam Part B Surveill. 2014;7(4):282-7. doi: 10.1080/19393210.2014.925981. Epub 2014 Jun 23.

DOI:10.1080/19393210.2014.925981

PMID:24867386

Abstract

Screening of zearalenone (ZEN)-producing species and monitoring of ZEN in feed were performed by using anti-zearalenone monoclonal antibodies. ELISA recoveries of ZEN from corn distillers dried grains with solubles (DDGS) feed, corn feed, rice bran, soybean meal, wheat bran and rapeseed dregs were between 78.6% and 88.6%. ZEN recovery from culture media was 96.3% at the spiked level of 500 µg/kg. Eighty-three samples of DDGS feed, corn feed and other fee ingredients were collected from 11 provinces of China and analysed for ZEN. Average ZEN levels were 563.4 µg/kg for DDGS feed, 393.1 µg/kg for corn feed and 65.7 µg/kg for other feed ingredients. Eighteen Fusarium species such as Fusarium graminearum which could produce ZEN were isolated from corn feed and other feed ingredients.

摘要

利用抗玉米赤霉烯酮单克隆抗体对产玉米赤霉烯酮（ZEN）的菌种进行筛选，并对饲料中的ZEN进行监测。从玉米干酒糟及其可溶物（DDGS）饲料、玉米饲料、米糠、豆粕、麦麸和菜籽粕中回收ZEN的酶联免疫吸附测定（ELISA）回收率在78.6%至88.6%之间。在500µg/kg的加标水平下，从培养基中回收ZEN的回收率为96.3%。从中国11个省份收集了83份DDGS饲料、玉米饲料和其他饲料原料样品，并对其进行ZEN分析。DDGS饲料中ZEN的平均含量为563.4µg/kg，玉米饲料中为393.1µg/kg，其他饲料原料中为65.7µg/kg。从玉米饲料和其他饲料原料中分离出18种能产生ZEN的镰刀菌，如禾谷镰刀菌。