Kavishankar G B, Moree S S, Lakshmidevi N
Department of Studies in Microbiology, University of Mysore, Mysore 570006, India; Department of Studies in Biochemistry, University of Mysore, Mysore 570006, India.
Department of Studies in Biochemistry, University of Mysore, Mysore 570006, India.
Phytomedicine. 2014 Jul-Aug;21(8-9):1026-31. doi: 10.1016/j.phymed.2014.04.033. Epub 2014 May 24.
To investigate the hepatoprotective, antioxidant and antihyperlipidemic effect of N-Trisaccharide isolated from Cucumis prophetarum (L.) on different experimental rats.
N-Trisaccharide (25 and 50 mg/kg.b.w), silymarin (25 mg/kg) and glibenclamide (25 mg/kg) was orally administered once daily for 28 days and toxicity evaluation studies were carried out. Liver damage was assessed by determining DNA damage, serum enzyme activities and hepatic histopathology of carbon tetrachloride (CCl4) induced hepatic injury in rats. Enzymatic and non enzymatic antioxidant levels in liver and kidney were determined and biochemical parameters such as, serum lipid profile, renal function markers were estimated in type 2 diabetic rats.
DNA fragmentation analysis revealed the protective effect of N-Trisaccharide on liver DNA damage. Histopathological studies indicated that CCl4-induced liver injury was less severe in N-Trisaccharide (25 and 50mg/kg) treated group. Given at the above doses conferred significant protection against the hepatotoxic actions of CCl4 in rats, reducing serum markers like SGOT, SGPT, ALP, creatinine and urea levels back to near normal (p<0.05) compared to untreated rats. In diabetic rats, N-Trisaccharide treatment significantly reversed abnormal status of enzymatic and non-enzymatic antioxidants levels to near normal. Also, serum lipids such as TG, TC, LDL-C and VLDL-C levels were significantly (p<0.05) reduced compared to diabetic untreated rats.
Present study results confirm that N-Trisaccharide possesses significant antihyperlipidemic, antioxidant and hepatoprotective properties.
研究从先知瓜（Cucumis prophetarum (L.)）中分离出的N-三糖对不同实验大鼠的肝脏保护、抗氧化和抗高血脂作用。
将N-三糖（25和50毫克/千克体重）、水飞蓟宾（25毫克/千克）和格列本脲（25毫克/千克）每日口服给药一次，持续28天，并进行毒性评估研究。通过测定大鼠四氯化碳（CCl4）诱导的肝损伤的DNA损伤、血清酶活性和肝脏组织病理学来评估肝损伤。测定肝脏和肾脏中的酶促和非酶促抗氧化水平，并估计2型糖尿病大鼠的生化参数，如血清脂质谱、肾功能指标。
DNA片段化分析显示N-三糖对肝脏DNA损伤具有保护作用。组织病理学研究表明，在N-三糖（25和50毫克/千克）治疗组中，CCl4诱导的肝损伤较轻。以上述剂量给药可显著保护大鼠免受CCl4的肝毒性作用，与未治疗的大鼠相比，使血清标志物如谷草转氨酶、谷丙转氨酶、碱性磷酸酶、肌酐和尿素水平恢复到接近正常（p<0.05）。在糖尿病大鼠中，N-三糖治疗显著将酶促和非酶促抗氧化剂水平的异常状态逆转至接近正常。此外，与未治疗的糖尿病大鼠相比，血清脂质如甘油三酯、总胆固醇、低密度脂蛋白胆固醇和极低密度脂蛋白胆固醇水平显著降低（p<0.05）。
本研究结果证实N-三糖具有显著的抗高血脂、抗氧化和肝脏保护特性。
