Chang Chih-Han, Chuang Shu-Chun, Wu Jia Hsing, Chen Jiun-Hong
Department of Earth and Planetary Sciences, Johns Hopkins University, 3400 N. Charles St., Baltimore, MD 21218, USA Department of Life Science, National Taiwan University, 1 Roosevelt Rd., Section 4, Taipei 106, Taiwan; Email:
Department of Life Science, National Taiwan University, 1 Roosevelt Rd., Section 4, Taipei 106, Taiwan Orthopaedic Research Center, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Kaohsiung 807, Taiwan; Email: unknown.
Zootaxa. 2014 Mar 7;3774:324-32. doi: 10.11646/zootaxa.3774.4.2.
The Metaphire formosae species group is a member of the Pheretima complex of the family Megascolecidae. It is composed of 12 nominal taxa, Metaphire bununa Tsai et al., 2000, Metaphire feijani Chang & Chen, 2004, Metaphire formosae (Michaelsen, 1922), Metaphire glareosa Tsai et al., 2000, Metaphire nanaoensis Chang & Chen, 2005, Metaphire paiwanna paiwanna Tsai et al., 2000, Metaphire paiwanna hengchunensis (James et al., 2005), Metaphire paiwanna liliumfordi Tsai et al., 2000, Metaphire tahanmonta Chang & Chen, 2005, Metaphire taiwanensis Tsai et al., 2004, Metaphire trutina Tsai et al., 2003, and Metaphire yuhsi (Tsai, 1964). In this study, we describe a new species, Metaphire tengjhihensis sp. nov., and two new subspecies, Metaphire nanaoensis truku ssp. nov. and Metaphire taiwanensis tsaii ssp. nov., belonging to this species group. DNA barcodes (partial sequences of the mitochondrial cytochrome c oxidase subunit 1, COI) from type specimens of M. feijani, M. tengjhihensis sp. nov., M. nanaoensis truku ssp. nov., M. tahanmonta and M. taiwanensis tsaii ssp. nov. have been deposited in GenBank in previous studies and are explicitly linked to the type specimens for the first time, enabling unambiguous identification using both morphology and DNA barcodes. Finally, we comment on the systematics of the M. formosae species group and suggest an integrative taxonomic approach that combines morphology and DNA barcodes for future descriptions of new species of Amynthas and Metaphire.
福尔摩沙巨蚓物种组是巨蚓科环毛蚓复合体的成员。它由12个命名分类单元组成，分别是蔡氏巨蚓（Metaphire bununa Tsai et al., 2000）、费氏巨蚓（Metaphire feijani Chang & Chen, 2004）、福尔摩沙巨蚓（Metaphire formosae (Michaelsen, 1922)）、光泽巨蚓（Metaphire glareosa Tsai et al., 2000）、南澳巨蚓（Metaphire nanaoensis Chang & Chen, 2005）、派湾巨蚓指名亚种（Metaphire paiwanna paiwanna Tsai et al., 2000）、派湾巨蚓恒春亚种（Metaphire paiwanna hengchunensis (James et al., 2005)）、派湾巨蚓李氏亚种（Metaphire paiwanna liliumfordi Tsai et al., 2000）、塔山巨蚓（Metaphire tahanmonta Chang & Chen, 2005）、台湾巨蚓（Metaphire taiwanensis Tsai et al., 2004）、校准巨蚓（Metaphire trutina Tsai et al., 2003）和玉里巨蚓（Metaphire yuhsi (Tsai, 1964)）。在本研究中，我们描述了一个新物种，即藤枝巨蚓（Metaphire tengjhihensis sp. nov.），以及两个新亚种，即南澳巨蚓特鲁库亚种（Metaphire nanaoensis truku ssp. nov.）和台湾巨蚓蔡氏亚种（Metaphire taiwanensis tsaii ssp. nov.），它们都属于这个物种组。在之前的研究中，费氏巨蚓、藤枝巨蚓新种、南澳巨蚓特鲁库亚种、塔山巨蚓和台湾巨蚓蔡氏亚种的模式标本的DNA条形码（线粒体细胞色素c氧化酶亚基1的部分序列，COI）已存入GenBank，并且首次与模式标本明确关联，这使得能够通过形态学和DNA条形码进行明确鉴定。最后，我们对福尔摩沙巨蚓物种组的系统学进行了评论，并提出了一种综合分类方法，该方法结合形态学和DNA条形码，用于未来对远盲蚓属（Amynthas）和巨蚓属（Metaphire）新物种的描述。
