Cheng Wei, Zhao Yu, Liu He, Fan Qin, Lu Fei-Fei, Li Jing, Yin Qin, Yan Chang-Dong
Affiliated Hospital of Xuzhou Medical College, 99 Huaihai West Road, Xuzhou, Jiangsu, 221002, China; Xuzhou medical College, Xuzhou, 221002, China; Jiangsu Province Key Laboratory of Anesthesiology and Center for Pain Research and Treatment, Xuzhou Medical College, Xuzhou, 221002, China.
Fundam Clin Pharmacol. 2014 Dec;28(6):661-70. doi: 10.1111/fcp.12084. Epub 2014 Aug 15.
The present study examined the effects of intrathecal use of resveratrol on pain hypersensitivities, spinal glia activation, and CX3CR1 expression in the model of bone cancer pain (BCP). The BCP model was established through intrathecally injecting Walker 256 mammary gland carcinoma cells to Sprague-Dawley rats. We found that spinal CX3CR1 expression and glial activation aggravated after inoculation. Resveratrol (i.t.) attenuated bone cancer-induced pain hypersensitivities, decreased CX3CR1 expression and glial activation in the spine in a BCP model. Resveratrol (i.t.) also attenuated mechanical allodynia resulting from intrathecally injecting fractalkine in rats. Inhibition of spinal glial activation and CX3CR1 upregulation may involve in resveratrol's analgesic effects. These findings demonstrated that resveratrol attenuated pain facilitation through inhibiting spinal glial activation and CX3CR1 upregulation in a BCP model.
本研究在骨癌痛（BCP）模型中检测了鞘内注射白藜芦醇对疼痛超敏反应、脊髓胶质细胞激活及CX3CR1表达的影响。通过向Sprague-Dawley大鼠鞘内注射Walker 256乳腺癌细胞建立BCP模型。我们发现接种后脊髓CX3CR1表达及胶质细胞激活加剧。在BCP模型中，白藜芦醇（鞘内注射）减轻了骨癌诱导的疼痛超敏反应，降低了脊髓中CX3CR1表达及胶质细胞激活。白藜芦醇（鞘内注射）还减轻了鞘内注射趋化因子诱导的大鼠机械性异常性疼痛。脊髓胶质细胞激活的抑制及CX3CR1上调可能与白藜芦醇的镇痛作用有关。这些发现表明，在BCP模型中，白藜芦醇通过抑制脊髓胶质细胞激活及CX3CR1上调减轻了疼痛易化。
