Lian Dong-Sheng, Zhao Shu-Jin
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China.
Analyst. 2014 Jul 21;139(14):3492-506. doi: 10.1039/c4an00400k.
Cancer is malignant disease that causes many deaths worldwide every year, with most deaths occurring in the middle and advanced stages of cancer. Numerous deaths can be avoided by detecting cancer at an early stage, making early diagnosis and timely therapy critical for cancer treatment. Analyses at the level of nucleic acids rather than phenotypes can eliminate various false-positive and -negative results, and diagnoses can occur at an earlier stage. Many techniques have been developed for this purpose, including capillary electrophoresis (CE), which has the advantages of high-efficiency, high-speed, high-throughput, automation, cleanliness, and versatility, and CE can be conducted on a microscale or coupled with other separation techniques. These advantages afford this technique the ability to meet the future medical requirements that will undoubtedly call for amassing large numbers of samples for analysis, suggesting that CE may become an important tool for providing data in clinical cancer diagnosis and therapy. This review focuses on CE-based nucleic acid detection as it is applied to cancer diagnosis and therapy, and provides an introduction to the drawbacks and future developments of analysis with CE.
癌症是一种恶性疾病，每年在全球导致许多人死亡，大多数死亡发生在癌症的中晚期。通过早期检测癌症可以避免许多死亡，因此早期诊断和及时治疗对癌症治疗至关重要。基于核酸水平而非表型的分析可以消除各种假阳性和假阴性结果，并且可以在更早阶段进行诊断。为此已经开发了许多技术，包括毛细管电泳（CE），它具有高效、高速、高通量、自动化、清洁和多功能等优点，并且CE可以在微尺度上进行或与其他分离技术联用。这些优点使该技术有能力满足未来的医学需求，而未来医学需求无疑需要大量样本进行分析，这表明CE可能成为临床癌症诊断和治疗中提供数据的重要工具。本综述重点关注基于CE的核酸检测在癌症诊断和治疗中的应用，并介绍CE分析的缺点和未来发展。
