Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an 710032, China.
Department of Gastroenterology, Shenyang General Hospital of PLA, Shenyang 110840, China.
Exp Biol Med (Maywood). 2014 Dec;239(12):1543-56. doi: 10.1177/1535370214533884. Epub 2014 May 28.
Accidental or intended radiation exposure in mass casualty settings presents a serious and on-going threat. The development of mitigating and treating agents requires appropriate animal models. Unfortunately, the majority of research on radiation enteritis in animals has lacked specific assessments and targeted therapy. Our study showed beagle dogs, treated by intensity-modulated radiation therapy (IMRT) for abdominal irradiation, were administered single X-ray doses of 8-30 Gy. The degree of intestinal tract injury for all of the animals after radiation exposure was evaluated with regard to clinical syndrome, endoscopic findings, histological features, and intestinal function. The range of single doses (8 Gy, 10-14 Gy, and 16-30 Gy) represented the degree of injury (mild, moderate, and severe, respectively). Acute radiation enteritis included clinical syndrome with fever, vomiting, diarrhea, hemafecia, and weight loss; typical endoscopic findings included edema, bleeding, mucosal abrasions, and ulcers; and intestinal biopsy results revealed mucosal necrosis, erosion, and loss, inflammatory cell infiltration, hemorrhage, and congestion. Changes in serum diamine oxides (DAOs) and d-xylose represented intestinal barrier function and absorption function, respectively, and correlated with the extent of damage (P < 0.05 and P < 0.05, respectively). We successfully developed a dog model of acute radiation enteritis, thus obtaining a relatively objective evaluation of intestinal tract injury based on clinical performance and laboratory examination. The method of assessment of the degree of intestinal tract injury after abdominal irradiation could be beneficial in the development of novel and effective therapeutic strategies for acute radiation enteritis.
在大规模突发事件中，意外或故意的辐射暴露带来了严重且持续存在的威胁。开发缓解和治疗药物需要适当的动物模型。然而，大多数关于动物辐射性肠炎的研究缺乏具体的评估和靶向治疗。我们的研究表明，接受腹部放射治疗的比格犬通过强度调制放射治疗（IMRT）进行治疗，给予单次 X 射线剂量 8-30Gy。所有动物在辐射暴露后的肠道损伤程度均通过临床综合征、内镜检查结果、组织学特征和肠道功能进行评估。单次剂量范围（8Gy、10-14Gy 和 16-30Gy）分别代表损伤程度（轻度、中度和重度）。急性放射性肠炎包括发热、呕吐、腹泻、血便和体重减轻的临床综合征；典型的内镜表现包括水肿、出血、黏膜磨损和溃疡；肠活检结果显示黏膜坏死、侵蚀和脱落、炎症细胞浸润、出血和充血。血清二胺氧化酶（DAO）和 D-木糖的变化分别代表肠道屏障功能和吸收功能，与损伤程度相关（P<0.05 和 P<0.05）。我们成功地建立了犬急性放射性肠炎模型，从而通过临床表现和实验室检查对肠道损伤获得了相对客观的评估。腹部照射后评估肠道损伤程度的方法可能有助于开发治疗急性放射性肠炎的新的有效治疗策略。
