Zhong Lu-Bin, Yin Jun, Zheng Yu-Ming, Liu Qing, Cheng Xiao-Xia, Luo Fang-Hong
Institute of Urban Environment, Chinese Academy of Sciences , 1799 Jimei Road, Xiamen 361021, P. R. China.
Anal Chem. 2014 Jul 1;86(13):6262-7. doi: 10.1021/ac404224f. Epub 2014 Jun 11.
We report a simple and rapid method for fabricating a surface-enhanced Raman scattering (SERS) substrate, which offers good flexibility, excellent optical transparency, and high SERS activity. Specifically, the SERS substrate (AuNPs/PMMA film) was obtained through self-assembly of gold nanoparticles (AuNPs) on newborn poly(methyl methacrylate) (PMMA) template. The UV-vis spectroscopy analysis and scanning electron microscopy observation revealed that the gold nanoparticles were closely assembled on the flexible and transparent PMMA template. The fabricated AuNPs/PMMA film SERS substrate allowed detection of model molecule, malachite green isothiocyanate, at a concentration as low as 0.1 nM, and exhibited good reproducibility in the SERS measurement. The Raman enhancement factor (EF) of the AuNPs/PMMA film was found to be as high as (2.4 ± 0.3) × 10(7). In addition, measure of residual malachite green on fish surface was carried out, and the result indicated that the AuNPs/PMMA film had great potential in the in situ ultrasensitive detection of analyte on irregular objects.
我们报道了一种用于制备表面增强拉曼散射（SERS）基底的简单快速方法，该基底具有良好的柔韧性、出色的光学透明度和高SERS活性。具体而言，SERS基底（金纳米颗粒/聚甲基丙烯酸甲酯薄膜）是通过金纳米颗粒（AuNPs）在新生的聚甲基丙烯酸甲酯（PMMA）模板上自组装获得的。紫外可见光谱分析和扫描电子显微镜观察表明，金纳米颗粒紧密组装在柔性透明的PMMA模板上。所制备的金纳米颗粒/聚甲基丙烯酸甲酯薄膜SERS基底能够检测低至0.1 nM浓度的模型分子异硫氰酸孔雀石绿，并且在SERS测量中表现出良好的重现性。发现金纳米颗粒/聚甲基丙烯酸甲酯薄膜的拉曼增强因子（EF）高达(2.4 ± 0.3) × 10(7)。此外，还对鱼表面的残留孔雀石绿进行了测量，结果表明金纳米颗粒/聚甲基丙烯酸甲酯薄膜在原位超灵敏检测不规则物体上的分析物方面具有巨大潜力。
