Ng Fong-Lee, Phang Siew-Moi, Periasamy Vengadesh, Yunus Kamran, Fisher Adrian C
Institute of Ocean and Earth Sciences, University of Malaya, Kuala Lumpur, Malaysia; Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
Low Dimensional Materials Research Centre, Department of Physics, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
PLoS One. 2014 May 29;9(5):e97643. doi: 10.1371/journal.pone.0097643. eCollection 2014.
In photosynthesis, a very small amount of the solar energy absorbed is transformed into chemical energy, while the rest is wasted as heat and fluorescence. This excess energy can be harvested through biophotovoltaic platforms to generate electrical energy. In this study, algal biofilms formed on ITO anodes were investigated for use in the algal biophotovoltaic platforms. Sixteen algal strains, comprising local isolates and two diatoms obtained from the Culture Collection of Marine Phytoplankton (CCMP), USA, were screened and eight were selected based on the growth rate, biochemical composition and photosynthesis performance using suspension cultures. Differences in biofilm formation between the eight algal strains as well as their rapid light curve (RLC) generated using a pulse amplitude modulation (PAM) fluorometer, were examined. The RLC provides detailed information on the saturation characteristics of electron transport and overall photosynthetic performance of the algae. Four algal strains, belonging to the Cyanophyta (Cyanobacteria) Synechococcus elongatus (UMACC 105), Spirulina platensis. (UMACC 159) and the Chlorophyta Chlorella vulgaris (UMACC 051), and Chlorella sp. (UMACC 313) were finally selected for investigation using biophotovoltaic platforms. Based on power output per Chl-a content, the algae can be ranked as follows: Synechococcus elongatus (UMACC 105) (6.38×10(-5) Wm(-2)/µgChl-a)>Chlorella vulgaris UMACC 051 (2.24×10(-5) Wm(-2)/µgChl-a)>Chlorella sp.(UMACC 313) (1.43×10(-5) Wm(-2)/µgChl-a)>Spirulina platensis (UMACC 159) (4.90×10(-6) Wm(-2)/µgChl-a). Our study showed that local algal strains have potential for use in biophotovoltaic platforms due to their high photosynthetic performance, ability to produce biofilm and generation of electrical power.
在光合作用中，所吸收的太阳能只有极少量转化为化学能，其余则以热能和荧光的形式被浪费掉。这种多余的能量可以通过生物光伏平台收集起来用于发电。在本研究中，对在ITO阳极上形成的藻类生物膜用于藻类生物光伏平台的情况进行了研究。筛选了16种藻类菌株，包括本地分离株和从美国海洋浮游植物培养保藏中心（CCMP）获得的两种硅藻，并基于悬浮培养的生长速率、生化组成和光合作用性能从中选出了8种。研究了这8种藻类菌株之间生物膜形成的差异，以及使用脉冲幅度调制（PAM）荧光计生成的快速光曲线（RLC）。RLC提供了有关藻类电子传递饱和特性和整体光合作用性能的详细信息。最终选择了4种藻类菌株用于生物光伏平台研究，它们分别属于蓝藻门（蓝细菌）的聚球藻（UMACC 105）、钝顶螺旋藻（UMACC 159），绿藻门的普通小球藻（UMACC 051）和小球藻属（UMACC 313）。基于每叶绿素a含量的功率输出，这些藻类的排名如下：聚球藻（UMACC 105）（6.38×10⁻⁵ Wm⁻²/μgChl-a）＞普通小球藻UMACC 051（2.24×10⁻⁵ Wm⁻²/μgChl-a）＞小球藻属（UMACC 313）（1.43×10⁻⁵ Wm⁻²/μgChl-a）＞钝顶螺旋藻（UMACC 159）（4.90×10⁻⁶ Wm⁻²/μgChl-a）。我们的研究表明，本地藻类菌株因其高光合作用性能、形成生物膜的能力和发电能力而具有用于生物光伏平台的潜力。
