Granholm A C, Dahl D, Siegel R A, Björklund H, Seiger A
Department of Histology, Karolinska Institutet, Stockholm, Sweden.
Department of Histology, Karolinska Institutet, Stockholm, Sweden; Department of Neuropathology, Harvard Medical School and Spinal Cord Injury Research Laboratory, West Roxbury Veterans Administration Medical Center, Boston, MA 02132, U.S.A.
Int J Dev Neurosci. 1985;3(2):149-56. doi: 10.1016/0736-5748(85)90005-X.
The influence of neonatal hypothyroidism on the development of immunoreactivity to glial fibrillary acidic protein (GFA) was studied in parietal cortex of rats treated from birth with the antithyroid agent propylthiouracil (PTU) for 3 or 8 weeks. Density of GFA immunoreactivity was evaluated in cryostate sections reacted with an antiserum specific for GFA. Three weeks postnatally, the density of GFA-immunoreactive structures in the cortical layers II-V was 70% lower in PTU-treated animals than in controls injected with the solvent. This marked difference between the groups was, however, not seen in either the molecular layer, layer VI or white matter. The inhibited development of GFA immunoreactivity was not persistent in animals treated with PTU for 8 weeks continuously. Plasma from animals treated with PTU for 1,2,3 and 8 weeks was collected and the TSH level in each group compared with samples from age-matched controls and newborn pups. The treatment with PTU resulted in a more than 10-fold increase in TSH level after 1 week of injections. In longterm groups of 8 weeks, the TSH level decreased in the PTU-treated animals, but stayed considerably higher than control values throughout the experiments. The results described in the present paper indicate a thyroid hormone dependent development of the GFA immunoreactivity in cortex cerebri astrocytes.
研究了新生期甲状腺功能减退对出生后即用抗甲状腺药物丙硫氧嘧啶（PTU）处理3周或8周的大鼠顶叶皮质中胶质纤维酸性蛋白（GFA）免疫反应性发育的影响。在与GFA特异性抗血清反应的冷冻切片中评估GFA免疫反应性的密度。出生后3周，PTU处理组动物皮质II-V层中GFA免疫反应性结构的密度比注射溶剂的对照组低70%。然而，在分子层、VI层或白质中，两组之间没有观察到这种显著差异。在连续用PTU处理8周的动物中，GFA免疫反应性的发育抑制并不持续。收集用PTU处理1、2、3和8周的动物的血浆，并将每组的促甲状腺激素（TSH）水平与年龄匹配的对照组和新生幼崽的样本进行比较。注射PTU 1周后，TSH水平增加了10倍以上。在8周的长期组中，PTU处理组动物的TSH水平下降，但在整个实验过程中仍显著高于对照值。本文所述结果表明，大脑皮质星形胶质细胞中GFA免疫反应性的发育依赖于甲状腺激素。
