Bayer S A
Department of Biology, Indiana-Purdue University, 1125 East 38th Street, Indianapolis, IN 46223, U.S.A.
Int J Dev Neurosci. 1985;3(3):229-43. doi: 10.1016/0736-5748(85)90028-0.
Neurogenesis in the magnocellular basal telencephalic nuclei of the rat was examined with [(3)H]thymidine autoradiography. The experimental animals were the offspring of pregnant females given two injections of [(3)H]thymidine on consecutive embryonic (E) days (E12-E13, E13-E14, … E21-E22). On postnatal day (P) 60, the percentage of labeled cells and the proportion of cells originating during 24 h periods were quantified at several anatomical levels throughout the magnocellular basal telencephalic nuclei. The neurons of the horizontal limb of the diagonal band originate mainly between E13 and E16 in a combined rostral-to-caudal and lateral-to-medial gradient. The neurogenetic gradients in the horizontal limb are continued by generation patterns of cells in the vertical limb of the diagonal band-medial septal complex, the large cells in the polymorph layer of the olfactory tubercle, and the large cells of the anterior amygdaloid area. The substantia innominata originates between E13 and E17 in combined caudal-to-rostral and lateral-to-medial gradients. The globus pallidus originates between E13 and E17 in combined caudal-to-rostral, ventral-to-dorsal and medial-to-lateral gradients. The entopeduncular nucleus originates between E12 and E14 in a 'sandwich' gradient where neurons in the core of the nucleus are older than those in either the anterior or posterior ends. There is an overall superficial (ventral) to deep (dorsal) neurogenetic gradient between the magnocellular basal nuclei present at any given rostrocaudal level. An important finding is that neurogenetic gradients in the individual components of the magnocellular basal nuclei are alike (with the possible exception of the entopeduncular nucleus) indicating they are part of a single system. Finally, evidence is presented that neurogenetic gradients in the magnocellular basal telencephalic neurons can be correlated with their anatomical projections to the cerebral cortex.
采用[³H]胸腺嘧啶核苷放射自显影术对大鼠大细胞性基底前脑核中的神经发生进行了研究。实验动物为怀孕母鼠的后代，这些母鼠在连续的胚胎期（E）第12 - 13天、第13 - 14天……第21 - 22天接受了两次[³H]胸腺嘧啶核苷注射。在出生后第60天（P60），在整个大细胞性基底前脑核的几个解剖层面上，对标记细胞的百分比以及在24小时时间段内产生的细胞比例进行了量化。斜角带水平支的神经元主要在胚胎期第13天至第16天之间，以从前到后和从外侧到内侧的联合梯度产生。斜角带水平支中的神经发生梯度通过斜角带 - 内侧隔复合体垂直支、嗅结节多形层中的大细胞以及杏仁前区的大细胞的产生模式得以延续。无名质在胚胎期第13天至第17天之间，以从后到前和从外侧到内侧的联合梯度产生。苍白球在胚胎期第13天至第17天之间，以从后到前、从腹侧到背侧和从内侧到外侧的联合梯度产生。内囊前核在胚胎期第12天至第14天之间，以一种“三明治”梯度产生，即核核心中的神经元比前端或后端的神经元更老。在任何给定的前后水平上，大细胞性基底核之间存在从表面（腹侧）到深部（背侧）的整体神经发生梯度。一个重要的发现是，大细胞性基底核各个组成部分中的神经发生梯度是相似的（内囊前核可能除外），这表明它们是一个单一系统的一部分。最后，有证据表明，大细胞性基底前脑神经元中的神经发生梯度与其向大脑皮层的解剖投射相关。
