Department of Chemical Engineering and Environmental Technology, Escuela de Ingenierías Industriales, Sede Dr. Mergelina, University of Valladolid, Dr. Mergelina s/n, 47011 Valladolid, Spain.
Socamex S.A.U (Grupo Urbaser), Polígono Industrial San Cristóbal, C/Cobalto 12, 47012 Valladolid, Spain.
Bioresour Technol. 2014 Jul;164:338-46. doi: 10.1016/j.biortech.2014.04.109. Epub 2014 May 9.
Biogas produced in an industrial-pilot scale sewage sludge reactor (5m(3)) was desulphurised by imposing microaerobic conditions. The H2S concentration removal efficiency was evaluated under various configurations: different mixing methods and O2 injection points. Biogas was entirely desulphurised under all the configurations set, while the O2 demand of the digester decreased over time. Although the H2S removal seemed to occur in the headspace, S(0) (which was found to be the main oxidation product) was scarcely deposited there in the headspace. O2 did not have a significant impact on the digestion performance; the VS removal remained around 47%. Conversely, DGGE revealed that the higher O2 transfer rate to the sludge maintained by biogas recirculation increased the microbial richness and evenness, and caused an important shift in the structure of the bacterial and the archaeal communities in the long term. All the archaeal genera identified (Methanosaeta, Methanospirillum and Methanoculleus) were present under both anaerobic and microaerobic conditions.
在工业中试规模的污水污泥反应器（5m(3))中产生的沼气通过施加微氧条件进行脱硫。在各种配置下评估了 H2S 浓度去除效率：不同的混合方法和 O2 注入点。在设定的所有配置下，沼气都被完全脱硫，而消化器中的 O2 需求随着时间的推移而减少。尽管 H2S 的去除似乎发生在气相中，但气相中几乎没有沉积 S(0)（这是发现的主要氧化产物）。O2 对消化性能没有显著影响；VS 去除率仍保持在 47%左右。相反，DGGE 显示，通过沼气再循环向污泥传递更高的 O2 转移率长期以来增加了微生物的丰富度和平坦度，并导致细菌和古菌群落结构发生重要变化。在厌氧和微氧条件下都存在鉴定出的所有古菌属（产甲烷菌、甲烷螺旋菌和甲烷微菌）。
