Nirmal J, Wolf-Johnston A S, Chancellor M B, Tyagi P, Anthony M, Kaufman J, Birder L A
Department of Urology, Oakland University William Beaumont School of Medicine, Rochester, MI, USA.
Int Urol Nephrol. 2014 Oct;46(10):1947-52. doi: 10.1007/s11255-014-0745-7. Epub 2014 May 30.
To study the protection offered by empty liposomes (LPs) alone against acrolein-induced changes in urothelial cell viability and explored uptake of LPs by primary (rat) urothelial cells.
Acrolein was used as a means to induce cellular damage and reduce urothelial cellular viability. The effect of acrolein or liposomal treatment on cellular proliferation was studied using 5-bromo-2'-deoxy-uridine assay. Cytokine release was measured after urothelial cells were exposed to acrolein. Temperature-dependent uptake study was carried out for fluorescent-labeled LPs using confocal microscopy.
Liposome pretreatment protected against acrolein-induced decrease in urothelial cell proliferation. LPs also significantly affected the acrolein-induced cytokine (interferon-gamma) release offering protection to the urothelial cells against acrolein damage. We also observed a temperature-dependent urothelial uptake of fluorescent-labeled LPs occurred at 37 °C (but not at 4 °C).
Empty LPs alone provide a therapeutic efficacy against acrolein-induced changes in urothelial cell viability and may be a promising local therapy for bladder diseases. Hence, our preliminary evidence provides support for liposome-therapy for urothelial protection and possible repair.
研究单独使用空脂质体（LPs）对丙烯醛诱导的尿路上皮细胞活力变化的保护作用，并探讨原代（大鼠）尿路上皮细胞对LPs的摄取情况。
使用丙烯醛诱导细胞损伤并降低尿路上皮细胞活力。采用5-溴-2'-脱氧尿苷测定法研究丙烯醛或脂质体处理对细胞增殖的影响。尿路上皮细胞暴露于丙烯醛后，测量细胞因子释放情况。使用共聚焦显微镜对荧光标记的LPs进行温度依赖性摄取研究。
脂质体预处理可保护尿路上皮细胞免受丙烯醛诱导的增殖减少。LPs还显著影响丙烯醛诱导的细胞因子（干扰素-γ）释放，为尿路上皮细胞提供保护，使其免受丙烯醛损伤。我们还观察到荧光标记的LPs在37℃时发生温度依赖性的尿路上皮摄取（4℃时未发生）。
单独的空LPs对丙烯醛诱导的尿路上皮细胞活力变化具有治疗效果，可能是一种有前景的膀胱疾病局部治疗方法。因此，我们的初步证据为脂质体治疗尿路上皮保护和可能的修复提供了支持。
