成年大鼠视网膜神经节细胞视神经挤压伤后髓鞘和淋巴细胞蛋白（MAL）的表达变化
The expression changes of myelin and lymphocyte protein (MAL) following optic nerve crush in adult rats retinal ganglion cells.
作者信息
Huang Yongsheng, Xu Yue, Cheng Qiaochu, Yu Shanshan, Gao Yi, Shu Qinmeng, Yang Cheng, Sun Yuan, Wang Jiawei, Xu Fan, Liang Xiaoling
机构信息
State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong Province, People's Republic of China.
出版信息
J Mol Neurosci. 2014 Dec;54(4):614-21. doi: 10.1007/s12031-014-0332-5. Epub 2014 May 31.
Myelin and lymphocyte protein (MAL), a component of compact myelin, is highly expressed in oligodendrocytes and Schwann cells. It has been reported that MAL may play a vital role in the process of neuronal apoptosis following acute spinal cord injury. However, acquaintance regarding its distribution and possible function in the retina is limited. Therefore, in a rodent model of optic nerve crush (ONC), the dynamic changes of MAL in retina was detected. The expression of MAL was mainly located in the retinal ganglion cells (RGCs) and was increased strongly after ONC. The peak of MAL expression appeared on the third day. In addition, there was a concomitant upregulation of active-caspase-3, which also co-localized with MAL in RGCs. Moreover, co-localization of MAL with terminal deoxynucleotidyl transferase-mediated biotinylated-dUTP nick-end labeling (TUNEL) was detected in RGCs after ONC. Collectively, all these results suggested that the upregulation of MAL might play an important role in the pathophysiology of RGCs after ONC.
髓鞘与淋巴细胞蛋白（MAL）是紧密髓鞘的一个组成部分，在少突胶质细胞和施万细胞中高度表达。据报道，MAL在急性脊髓损伤后的神经元凋亡过程中可能起着至关重要的作用。然而，关于其在视网膜中的分布及可能功能的了解有限。因此，在视神经挤压（ONC）的啮齿动物模型中，检测了视网膜中MAL的动态变化。MAL的表达主要位于视网膜神经节细胞（RGCs），ONC后其表达强烈增加。MAL表达的峰值出现在第三天。此外，活性半胱天冬酶-3也伴随上调，且在RGCs中也与MAL共定位。而且，ONC后在RGCs中检测到MAL与末端脱氧核苷酸转移酶介导的生物素化-dUTP缺口末端标记（TUNEL）共定位。总的来说，所有这些结果表明，MAL的上调可能在ONC后RGCs的病理生理学中起重要作用。
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