8-甲氧基补骨脂素对兔角膜内皮的光毒性作用。

Phototoxic effects of 8-methoxypsoralen on rabbit corneal endothelium.

作者信息

Menon I A, Basu P K, Hasany S M, Persad S D

机构信息

Department of Ophthalmology, University of Toronto, On, Canada.

出版信息

Lens Eye Toxic Res. 1989;6(1-2):289-300.

PMID:2488022

Abstract

The phototoxic effects of 8-methoxypsoralen (8-MOP) were investigated using the rabbit corneal endothelium in organ culture. The corneas were divided into four groups: (a) irradiated with a mercury vapor lamp (emitting UVA and visible radiation) in the presence of 8-MOP (experimental), (b) irradiated without 8-MOP (control A), (c) incubated with 8-MOP (control B) and (d) incubated without 8-MOP (control C). Specular and light microscopic examination showed that the experimental corneas had greater cellular damage compared to the control corneas. The effects of 8-MOP were restricted to certain localized areas of the cornea. However there was no significant difference in the amounts of 51Cr released from the labelled experimental and control corneas. These results show phototoxic damage of the corneal endothelial cells.

摘要

利用器官培养的兔角膜内皮研究了8-甲氧基补骨脂素（8-MOP）的光毒性作用。角膜被分为四组：（a）在8-MOP存在的情况下用汞蒸气灯照射（发射紫外线A和可见光）（实验组），（b）无8-MOP照射（对照A），（c）与8-MOP孵育（对照B），以及（d）无8-MOP孵育（对照C）。镜面反射和光学显微镜检查显示，与对照角膜相比，实验角膜的细胞损伤更大。8-MOP的作用局限于角膜的某些局部区域。然而，标记的实验角膜和对照角膜释放的51Cr量没有显著差异。这些结果表明角膜内皮细胞存在光毒性损伤。