一种用于从乳制品副产物生物催化生产 3'-唾液乳糖的集成膜系统。
An integrated membrane system for the biocatalytic production of 3'-sialyllactose from dairy by-products.
机构信息
Department of Chemical and Biochemical Engineering, Center for BioProcess Engineering, Building 229, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
Department of Chemical and Biochemical Engineering, Center for BioProcess Engineering, Building 229, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
出版信息
Bioresour Technol. 2014 Aug;166:9-16. doi: 10.1016/j.biortech.2014.05.003. Epub 2014 May 10.
An integrated membrane system was investigated for the production of 3'-sialyllactose by an engineered sialidase using casein glycomacropeptide (CGMP) and lactose as substrates. CGMP was purified by ultrafiltration (UF) to remove any small molecules present and then an enzymatic membrane reactor (EMR) was used to separate the product and reuse the enzyme. A PLCC regenerated cellulose membrane was found to be the most suitable for both the UF purification and EMR. Subsequently, nanofiltration (NF) was conducted to increase the purity of the 3'-sialyllactose by removing the excess lactose present. The NTR7450 membrane outperformed others in NF due to its high retention of 3'-sialyllactose (98%) and relatively low rejection of lactose (40%). The lactose in the permeate could be concentrated by the NF45 membrane and recycled into the EMR. The described integrated membrane system enables a more economic and efficient enzymatic production of 3'-sialyllactose.
采用工程化唾液酸酶，以酪蛋白糖巨肽（CGMP）和乳糖为底物，研究了一种集成膜系统用于 3'-唾液酸乳糖的生产。通过超滤（UF）对 CGMP 进行纯化，以去除任何存在的小分子，然后使用酶膜反应器（EMR）分离产物并重复使用酶。PLCC 再生纤维素膜被发现最适合 UF 纯化和 EMR。随后，通过纳滤（NF）去除多余的乳糖，以提高 3'-唾液酸乳糖的纯度。NTR7450 膜在 NF 中表现出色，因为它对 3'-唾液酸乳糖的保留率高（98%），而对乳糖的排斥率相对较低（40%）。NF45 膜可浓缩透过液中的乳糖，并回收至 EMR。所描述的集成膜系统使 3'-唾液酸乳糖的酶法生产更经济、更高效。
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