[甲状旁腺激素与雌激素对去势雌性大鼠牙槽骨代谢的影响]

[Influence of parathyroid hormone and estrogen on alveolar bone metabolism of castrated female rats].

作者信息

Sun Zhe, Lin Zhiyong, Bai Guangliang, Di Jing, Jiang Lixia

出版信息

Hua Xi Kou Qiang Yi Xue Za Zhi. 2014 Apr;32(2):134-7. doi: 10.7518/hxkq.2014.02.007.

DOI:10.7518/hxkq.2014.02.007

PMID:24881206

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7030809/

Abstract

OBJECTIVE

To investigate the influence of parathyroid hormone and estrogen on alveolar bone metabolism of castrated female rats.

METHODS

Sixty-six female Wistar rats which were healthy and 4 months old were divided into two groups, with group SHAM (n = 18) and group ovariectomy (OVX) (n = 48). After 8 weeks of ovariectomy, the osteoporosis model was confirmed by examing 8 ovariectomized and sham-operated rats. The rest 10 rats in group SHAM were the control group (group A). The rest 40 rats in group OVX were divided into ovariectomized group (group B), ovariectomized and treated with estrogen (group C), ovariectomized and treated with parathyroid hormone (group D), ovariectomized and treated with estrogen and parathyroid hormone (group E) at random with 10 in each group. Group A and B injected physiological saline (1 mL x kg(-1)), group C injected estradiol benzoate (10 microg x kg(-1)), group D injected parathyroid hormone (20 microg x kg(-1)), group E injected parathyroid hormone (20 microg x kg(-1)) and estradiol benzoate (10 microg x kg(-1)). The intraperitoneal injection were maken every other day to rats in each group, which continued for 8 weeks. The bone mineral density (BMD), bone histomorphology and serum Ca, P, alkaline phosphatase (ALP) were measured after therapy.

RESULTS

After 8 weeks of ovariectomy, the lumbar BMD of ovariectomized rats were significantly declined compared with those of the sham-operated rats (P < 0.05). Eight weeks later after the drug use, the BMD, %Tb.Ar, Tb.Th, Tb.N in group C, D, E were slightly elevated compared to group B, especially the group E (P < 0.05). Serum calcium and phosphorus values did not change significantly (P > 0.05). ALP values in group B was significantly higher than that in group A (P < 0.05).

CONCLUSION

Intermittent application of parathyroid hormone in small doses can increase alveolar BMD of castration rats and improve their bone structure. And it can have synergy effects on the treatment of osteoporosis if it is used combining with estrogen.

摘要

目的

探讨甲状旁腺激素和雌激素对去势雌性大鼠牙槽骨代谢的影响。

方法

选取66只4月龄健康雌性Wistar大鼠，分为假手术组（SHAM，n = 18）和去卵巢组（OVX，n = 48）。去卵巢8周后，通过检测8只去卵巢大鼠和假手术大鼠确认骨质疏松模型。SHAM组剩余10只大鼠为对照组（A组）。OVX组剩余40只大鼠随机分为去卵巢组（B组）、去卵巢并用雌激素治疗组（C组）、去卵巢并用甲状旁腺激素治疗组（D组）、去卵巢并用雌激素和甲状旁腺激素治疗组（E组），每组10只。A组和B组注射生理盐水（1 mL·kg⁻¹），C组注射苯甲酸雌二醇（10 μg·kg⁻¹），D组注射甲状旁腺激素（20 μg·kg⁻¹），E组注射甲状旁腺激素（20 μg·kg⁻¹）和苯甲酸雌二醇（10 μg·kg⁻¹）。每组大鼠隔日进行腹腔注射，持续8周。治疗后检测骨密度（BMD）、骨组织形态学及血清钙、磷、碱性磷酸酶（ALP）。

结果

去卵巢8周后，去卵巢大鼠腰椎BMD较假手术大鼠显著下降（P < 0.05）。用药8周后，C组、D组、E组的BMD、骨小梁面积百分比（%Tb.Ar）、骨小梁厚度（Tb.Th）、骨小梁数量（Tb.N）较B组略有升高，尤其是E组（P < 0.05）。血清钙和磷值无明显变化（P > 0.05）。B组ALP值显著高于A组（P < 0.05）。