土壤纤毛虫 Kahliella matisi（纤毛门、下口目）中的端粒重复序列和巨核 DNA 组织。
Telomere repeats and macronuclear DNA organization in the soil ciliate Kahliella matisi (Ciliophora, Hypotricha).
机构信息
Associated Tissue Bank of Faculty of Medicine of P. J. Šafárik University and L. Pasteur University Hospital, Trieda SNP 1, 041 66 Košice, Slovakia.
Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4-6, 040 01 Košice, Slovakia.
出版信息
Eur J Protistol. 2014 Jun;50(3):231-5. doi: 10.1016/j.ejop.2014.03.002. Epub 2014 Mar 18.
To better understand the structure of macronuclear chromosomes in ciliates, the organization of macronuclear DNA was investigated in the hypotrich Kahliella matisi. Total DNA of K. matisi separated by agarose gel electrophoresis showed continuous smear ranging in size from ∼500bp to ∼15kb. This fragmentation was found to be due to the presence of gene-sized macronuclear chromosomes. The sequence analysis of four randomly cloned macronuclear chromosomes showed that K. matisi telomeres consist of 5'-dC4A4-3' repeats and carry one or two open reading frames. The transcription unit was found to be flanked with non-coding AT rich 5' leader and 3' trailer. No consensus transcription-regulatory sequences were identified in 5' leader and only one of analyzed gene-sized chromosomes showed the presence of conserved poly(A) addition signal sequence in 3' trailer. All ORFs showed highest relatedness to Oxytricha trifallax macronuclear chromosomes with conserved exon/intron structure. Sequence comparisons indicate that macronuclear chromosome organization is at least partially conserved in ciliates.
为了更好地了解纤毛虫的巨核染色体结构，对 Hypotrich 中的 Kahliella matisi 的巨核 DNA 组织进行了研究。琼脂糖凝胶电泳分离的 K. matisi 的总 DNA 显示出大小从约 500bp 到约 15kb 的连续弥散。这种碎片化被发现是由于存在基因大小的巨核染色体所致。对四个随机克隆的巨核染色体的序列分析表明，K. matisi 的端粒由 5'-dC4A4-3'重复组成，并携带一个或两个开放阅读框。转录单元被侧翼非编码的 AT 丰富的 5' 先导和 3' 尾随。在 5' 先导中没有鉴定出一致的转录调控序列，只有一个分析的基因大小的染色体在 3' 尾随中显示出保守的多（A）添加信号序列的存在。所有 ORF 与 Oxytricha trifallax 的巨核染色体表现出最高的同源性，具有保守的外显子/内含子结构。序列比较表明，纤毛虫的巨核染色体组织至少部分保守。
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