一种来自灵芝的免疫调节蛋白（Ling Zhi-8）可促进电外科单极手术治疗后大鼠肝脏组织的伤口愈合。
An Immunomodulatory Protein (Ling Zhi-8) from a Ganoderma lucidum Induced Acceleration of Wound Healing in Rat Liver Tissues after Monopolar Electrosurgery.
机构信息
School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan ; Research Center for Biomedical Implants and Microsurgery Devices, Taipei Medical University, Taipei 110, Taiwan.
Division of Oral Rehabilitation and Center of Pediatric Dentistry, Department of Dentistry, Taipei Medical University Hospital, Taipei 110, Taiwan ; Research Center for Biomedical Devices and Prototyping Production, Taipei Medical University, Taipei 110, Taiwan.
出版信息
Evid Based Complement Alternat Med. 2014;2014:916531. doi: 10.1155/2014/916531. Epub 2014 May 5.
The purpose of this study was to investigate the effect of an immunomodulatory protein (Ling Zhi-8, LZ-8) on wound healing in rat liver tissues after monopolar electrosurgery. Animals were sacrificed for evaluations at 0, 3, 7, and 28 days postoperatively. It was found that the wound with the LZ-8 treatment significantly increases wound healing. Western blot analysis clearly indicated that the expression of NF-κB was decreased at 3, 7, and 28 days when liver tissues were treated with LZ-8. Moreover, caspase-3 activity of the liver tissue also significantly decreases at 7 and 28 days, respectively. DAPI staining and TUNEL assays revealed that only a minimal dispersion of NF-κB was found on the liver tissue treated with LZ-8 at day 7 as compared with day 3 and tissues without LZ-8 treatment. Similarly, apoptosis was decreased on liver tissues treated with LZ-8 at 7 days when compared to the control (monopolar electrosurgery) tissues. Therefore, the analytical results demonstrated that LZ-8 induced acceleration of wound healing in rat liver tissues after monopolar electrosurgery.
本研究旨在探讨免疫调节蛋白（灵芝-8，LZ-8）对单极电外科术后大鼠肝组织伤口愈合的影响。动物在术后 0、3、7 和 28 天被处死以进行评估。结果发现，LZ-8 治疗的伤口明显增加了伤口愈合。Western blot 分析清楚地表明，当用 LZ-8 处理肝组织时，NF-κB 的表达在 3、7 和 28 天时降低。此外，肝组织中 caspase-3 的活性在第 7 和第 28 天也分别显著降低。DAPI 染色和 TUNEL 分析显示，与第 3 天和未用 LZ-8 处理的组织相比，用 LZ-8 处理的肝组织中 NF-κB 的弥散仅最小。同样，与对照组（单极电外科）组织相比，在第 7 天用 LZ-8 处理的肝组织中凋亡减少。因此，分析结果表明，LZ-8 诱导了单极电外科术后大鼠肝组织伤口愈合的加速。
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