Department of Biology and Center for Biodiversity, East Carolina University , Greenville, NC , USA.
Department of Food Science, College of Agriculture and Life Sciences, Cornell University , Ithaca, NY , USA ; Department of Neurobiology, Physiology and Behavior, University of California , Davis, CA , USA.
PeerJ. 2014 May 22;2:e396. doi: 10.7717/peerj.396. eCollection 2014.
Emberizid sparrows (emberizidae) have played a prominent role in the study of avian vocal communication and social behavior. We present here brain transcriptomes for three emberizid model systems, song sparrow Melospiza melodia, white-throated sparrow Zonotrichia albicollis, and Gambel's white-crowned sparrow Zonotrichia leucophrys gambelii. Each of the assemblies covered fully or in part, over 89% of the previously annotated protein coding genes in the zebra finch Taeniopygia guttata, with 16,846, 15,805, and 16,646 unique BLAST hits in song, white-throated and white-crowned sparrows, respectively. As in previous studies, we find tissue of origin (auditory forebrain versus hypothalamus and whole brain) as an important determinant of overall expression profile. We also demonstrate the successful isolation of RNA and RNA-sequencing from post-mortem samples from building strikes and suggest that such an approach could be useful when traditional sampling opportunities are limited. These transcriptomes will be an important resource for the study of social behavior in birds and for data driven annotation of forthcoming whole genome sequences for these and other bird species.
燕雀科鸟类（Emberizidae）在研究鸟类的声音交流和社会行为方面发挥了重要作用。我们在这里展示了三种燕雀科模型系统的大脑转录组，即歌雀（Melospiza melodia）、白喉雀（Zonotrichia albicollis）和金冠戴菊鸟（Zonotrichia leucophrys gambelii）。每个组装完全或部分覆盖了斑胸草雀（Taeniopygia guttata）中以前注释的蛋白编码基因的 89%以上，在歌雀、白喉雀和金冠戴菊鸟中分别有 16846、15805 和 16646 个独特的 BLAST 命中。与以前的研究一样，我们发现组织起源（听觉前脑与下丘脑和全脑）是整体表达谱的一个重要决定因素。我们还证明了从建筑撞击后的死后样本中成功分离 RNA 和 RNA 测序，并表明在传统采样机会有限时，这种方法可能很有用。这些转录组将是研究鸟类社会行为以及为这些和其他鸟类的全基因组序列进行数据驱动注释的重要资源。
