室内灰尘中麦角固醇水平的决定因素、可重复性和季节性变化。

Determinants, reproducibility, and seasonal variation of ergosterol levels in house dust.

出版信息

Indoor Air. 2014 Jun;24(3):248-59. doi: 10.1111/ina.12078.

DOI:10.1111/ina.12078

Abstract

UNLABELLED

This study aimed to clarify the determinants that affect the concentrations of ergosterol and viable fungi in house dust and to examine the seasonal variation and reproducibility of ergosterol concentrations indoors. In studying the determinants, dust samples from living room floors and vacuum cleaner dust bags were collected from 107 farming and 105 non-farming homes. Ergosterol levels were determined with gas chromatography-mass spectrometry,and the dust bag dust was cultivated for enumeration of fungal genera. Lifestyle and environmental factors, for example using of the fireplace, and visible mold observations in homes, explained 20–26% of the variation of fungal concentrations. For the reproducibility study, samples were collected from five urban homes in four different seasons. The reproducibility of ergosterol determinations within a sample was excellent (ICC = 89.8) for floor dust and moderate (ICC = 63.8) for dust bag dust, but poor when sampling the same home throughout a year (ICC = 31.3 and 12.6, respectively) due to large temporal variation in ergosterol concentrations. In conclusion, environmental characteristics only partially predicted the variation of fungal concentrations. Based on these studies, we recommend repeated sampling of dust over time if one seeks to adequately describe overall fungal levels and exposure in a home.

PRACTICAL IMPLICATIONS

This study shows that levels of ergosterol and viable fungi in house dust are related to visible mold observations. Only 20% of the variation in fungal levels can be explained with questionnaires, and therefore, environmental samples need to be taken in addition. Reproducibility of ergosterol determination was excellent for floor dust, and thus, ergosterol measurements from floor dust samples could be suitable for assessing the fungal load in building investigations. The temporal variation needs to be taken into account when describing the ergosterol concentration of urban homes.

摘要

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本研究旨在阐明影响室内灰尘中麦角固醇和真菌浓度的决定因素，并研究室内麦角固醇浓度的季节性变化和可重复性。在研究决定因素时，从 107 个农场家庭和 105 个非农场家庭的客厅地板灰尘和真空吸尘器尘袋中收集了灰尘样本。采用气相色谱-质谱法测定麦角固醇水平，并用尘袋灰尘培养真菌属进行计数。生活方式和环境因素，例如使用壁炉和家中可见霉菌的观察，解释了真菌浓度变化的 20-26%。为了进行可重复性研究，从四个不同季节的五个城市家庭中采集了样本。地板灰尘的麦角固醇测定值的内部样本重复性非常好（ICC=89.8），而尘袋灰尘的中等（ICC=63.8），但由于麦角固醇浓度的时间变化很大，全年对同一家庭进行采样的可重复性较差（ICC 分别为 31.3 和 12.6）。总之，环境特征仅部分预测了真菌浓度的变化。基于这些研究，如果要充分描述家中的总体真菌水平和暴露情况，我们建议随着时间的推移重复采集灰尘样本。