豆科植物种子贮藏蛋白的进化——豆球蛋白和豌豆球蛋白共有的一个结构域在豌豆球蛋白中发生了重复。

Evolution of legume seed storage proteins--a domain common to legumins and vicilins is duplicated in vicilins.

作者信息

Gibbs P E, Strongin K B, McPherson A

机构信息

Department of Biochemistry, University of California, Riverside 92521.

出版信息

Mol Biol Evol. 1989 Nov;6(6):614-23. doi: 10.1093/oxfordjournals.molbev.a040575.

DOI:10.1093/oxfordjournals.molbev.a040575

PMID:2488475

Abstract

We examined the primary sequence of canavalin, the major storage protein of jack beans, and found that an ancient sequence duplication accounts for 80% of the amino acid residues. Evidence for such a duplication was also found in the orthologous proteins phaseolin and pea vicilin. This sequence duplication presumably accounts for a structural duplication in the canavalin monomer observed by crystallographic analysis. One copy of this repeat was found in a second storage-protein family, the legumins, where it encompasses almost the entire B-chain of the mature molecule. We propose that the vicilin and legumin families of legume seed proteins evolved from a common precursor, which consisted of one copy of the repeat in the vicilins.

摘要

我们研究了刀豆球蛋白（菜豆的主要储存蛋白）的一级序列，发现一个古老的序列重复占氨基酸残基的80%。在直系同源蛋白菜豆蛋白和豌豆豆球蛋白中也发现了这种重复的证据。这种序列重复可能是晶体学分析中观察到的刀豆球蛋白单体结构重复的原因。在第二个储存蛋白家族豆球蛋白中发现了该重复序列的一个拷贝，它几乎涵盖了成熟分子的整个B链。我们提出，豆科植物种子蛋白的豆球蛋白和豆球蛋白家族是由一个共同的前体进化而来的，该前体在豆球蛋白中由重复序列的一个拷贝组成。