光介导的丝状真菌里氏木霉基因表达调控
Light-mediated control of gene expression in filamentous fungus Trichoderma reesei.
作者信息
Wang Wei, Shi Xiang-Yu, Wei Dong-Zhi
机构信息
State key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.
State key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.
出版信息
J Microbiol Methods. 2014 Aug;103:37-9. doi: 10.1016/j.mimet.2014.05.017. Epub 2014 Jun 2.
We developed a light-mediated system based on synthetic light-switchable transactivators. The transactivators bind promoter upon blue-light exposure and rapidly initiate transcription of target transgenes in filamentous fungus Trichoderma reesei. Light is inexpensive to apply, easily delivered, and instantly removed, and thus has significant advantages over chemical inducers.
我们基于合成的光可切换反式激活因子开发了一种光介导系统。这些反式激活因子在蓝光照射下与启动子结合，并在丝状真菌里氏木霉中迅速启动靶标转基因的转录。光的应用成本低廉、易于传递且可即时去除，因此与化学诱导剂相比具有显著优势。
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