活细胞上位点特异性标记的转化生长因子II型受体的单分子动力学
Single-molecule dynamics of site-specific labeled transforming growth factor type II receptors on living cells.
作者信息
Cheng Ming, Zhang Wei, Yuan Jinghe, Luo Wangxi, Li Nan, Lin Shixian, Yang Yi, Fang Xiaohong, Chen Peng R
机构信息
Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Key Laboratory of Molecular Nanostructures and Nanotechnology, Chinese Academy of Sciences, Beijing 100190, P. R. China.
出版信息
Chem Commun (Camb). 2014 Dec 7;50(94):14724-7. doi: 10.1039/c4cc02804j.
We achieved single-molecule imaging and tracking of the transforming growth factor type II receptor (TβRII) that was labeled by an organic dye via a genetically encoded unnatural amino acid (UAA) and the copper-free click chemistry. The stoichiometry, mobility and dimerization kinetics of individual TβRII molecules were determined.
我们通过基因编码的非天然氨基酸（UAA）和无铜点击化学实现了对用有机染料标记的转化生长因子II型受体（TβRII）的单分子成像和追踪。确定了单个TβRII分子的化学计量、迁移率和二聚化动力学。
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