Liu Yang, Wei Zhuying, Huang Yafei, Bai Chunling, Zan Linsen, Li Guangpeng
College of Animal Science and Technology, Northwest A&F University, Yangling, China.
Anim Sci J. 2014 Sep;85(9):840-7. doi: 10.1111/asj.12220. Epub 2014 Jun 3.
Hedgehog (Hh) pathway has been studied in various animal body life procedures and is suggested to be important for the development of multiple organs. The genes involved in the Hh signaling pathway were expressed in the ovary of mice, pigs and cattle. However, the function of Hh signaling pathway on oocyte maturation and early embryonic development is still controversial. We detected the effect of sonic hedgehog (Shh) and cyclopamine on the in vitro maturation of mouse oocytes and embryo development. The results showed that the presence of Shh or cyclopamine resulted in similar oocyte maturation to control groups. Shh did not improve early embryonic development. However, the supplement of cyclopamine depressed early embryo development. The mRNA of shh, ptch1, smo and gli1 were less detected in the denuded oocytes. The expression levels of ptch1 ascended from the uncleaved zygote to blastocyst stage. Smo or gli1 were expressed on a higher level at the two-cell or four-cell stage in early embryonic development separately. Therefore, Shh did not affect mouse oocyte maturation and early embryo development, but cyclopamine led to inhibited development of mouse early embryo. The effects of Hh signaling on the oocyte maturation and early embryo development might be species-specific.
刺猬（Hh）信号通路已在动物身体的各种生命过程中得到研究，并且被认为对多个器官的发育很重要。参与Hh信号通路的基因在小鼠、猪和牛的卵巢中表达。然而，Hh信号通路在卵母细胞成熟和早期胚胎发育中的作用仍存在争议。我们检测了音猬因子（Shh）和环杷明对小鼠卵母细胞体外成熟和胚胎发育的影响。结果表明，Shh或环杷明的存在导致卵母细胞成熟情况与对照组相似。Shh并未改善早期胚胎发育。然而，添加环杷明会抑制早期胚胎发育。在裸卵中较少检测到shh、ptch1、smo和gli1的mRNA。ptch1的表达水平从未分裂的合子到囊胚期逐渐上升。在早期胚胎发育中，smo或gli1分别在二细胞期或四细胞期表达水平较高。因此，Shh不影响小鼠卵母细胞成熟和早期胚胎发育，但环杷明会导致小鼠早期胚胎发育受到抑制。Hh信号对卵母细胞成熟和早期胚胎发育的影响可能具有物种特异性。
