Zhou Lu, Sohet Fabien, Daneman Richard
Department of Neurobiology, Stanford University School of Medicine, Stanford, California 94305-5125.
Department of Anatomy, UCSF, San Francisco, California 94143-0452.
Cold Spring Harb Protoc. 2014 Jun 2;2014(6):581-3. doi: 10.1101/pdb.top070888.
Pericytes are found on the abluminal surface of endothelial tubes. The function of these cells is still being elucidated, but they have been shown to be important for development and maintenance of the blood-brain barrier, regulation of angiogenesis and capillary blood flow, and regulation of the neural response to injury and disease. Previously used methods to isolate pericytes have relied on negative selection and prolonged culture of microvessel cells and may lead to populations of pericytes contaminated by other neural cell types. We have developed an immunopanning protocol to specifically purify pericytes from capillaries in the rodent optic nerve. This method relies on a combination of negative and positive selection criteria and allows prospective, acute isolation of pericytes. Use of this method will facilitate studies of pericyte cell biology and function and pericyte-endothelial cell interactions.
周细胞存在于内皮管的无腔表面。这些细胞的功能仍在研究中，但已证明它们对于血脑屏障的发育和维持、血管生成和毛细血管血流的调节以及神经对损伤和疾病的反应调节很重要。以前用于分离周细胞的方法依赖于微血管细胞的阴性选择和长时间培养，可能导致周细胞群体被其他神经细胞类型污染。我们开发了一种免疫淘选方案，以从啮齿动物视神经的毛细血管中特异性纯化周细胞。该方法依赖于阴性和阳性选择标准的组合，并允许前瞻性、急性分离周细胞。使用该方法将有助于周细胞生物学和功能以及周细胞 - 内皮细胞相互作用的研究。
