通过扫描隧道显微镜揭示的球蛋白豌豆球蛋白的结构。

Structure of the globular protein vicilin revealed by scanning tunnelling microscopy.

作者信息

Welland M E, Miles M J, Lambert N, Morris V J, Coombs J H, Pethica J B

机构信息

Department of Engineering, Cambridge, UK.

出版信息

Int J Biol Macromol. 1989 Feb;11(1):29-32. doi: 10.1016/0141-8130(89)90036-6.

DOI:10.1016/0141-8130(89)90036-6

PMID:2489056

Abstract

Scanning tunnelling microscopy (s.t.m.) has been used to study the structure of the non-crystalline globular protein vicilin. Molecules were deposited on amorphous carbon substrates and imaged both in air and in vacuo without additional sample preparation. Current-voltage plots of an individual protein molecule are also reported. The s.t.m. images are compared with conventional transmission electron micrographs and with a model of vicilin based on small-angle synchrotron X-ray scattering data.

摘要

扫描隧道显微镜（STM）已被用于研究非晶态球状蛋白豌豆球蛋白的结构。将分子沉积在非晶碳基底上，无需额外的样品制备，就在空气和真空中进行成像。还报道了单个蛋白质分子的电流-电压曲线。将STM图像与传统透射电子显微镜图像以及基于小角同步加速器X射线散射数据的豌豆球蛋白模型进行了比较。