亲水性、明亮的硫化铜铟量子点作为无镉荧光标记物用于定量免疫分析。
Hydrophilic, bright CuInS2 quantum dots as Cd-free fluorescent labels in quantitative immunoassay.
作者信息
Speranskaya Elena S, Beloglazova Natalia V, Abé Sofie, Aubert Tangi, Smet Philippe F, Poelman Dirk, Goryacheva Irina Y, De Saeger Sarah, Hens Zeger
机构信息
Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University , Harelbekestraat 72, 9000 Gent, Belgium.
出版信息
Langmuir. 2014 Jul 1;30(25):7567-75. doi: 10.1021/la501268b. Epub 2014 Jun 16.
We report on the synthesis of core-shell CuInS2/ZnS quantum dots (QDs) in organic solution, their encapsulation with a PEG-containing amphiphilic polymer, and the application of the resulting water-soluble QDs as fluorescent label in quantitative immunoassay. By optimizing the methods for core synthesis and shell growth, CuInS2/ZnS QDs were obtained with a quantum yield of 50% on average after hydrophilization. After conjugation with an aflatoxin B1-protein derivative, the obtained QDs were used as fluorescent labels in microplate immunoassay for the quantitative determination of the mycotoxin aflatoxin B1. QDs-based immunoassay showed higher sensitivity compared to enzyme-based immunoassay.
我们报道了在有机溶液中合成核壳结构的CuInS2/ZnS量子点（QDs），用含聚乙二醇的两亲聚合物对其进行封装，以及将所得水溶性量子点用作荧光标记物进行定量免疫分析。通过优化核合成和壳生长的方法，亲水化后平均量子产率为50%的CuInS2/ZnS量子点得以制备。与黄曲霉毒素B1 - 蛋白质衍生物偶联后，所得量子点被用作微孔板免疫分析中的荧光标记物，用于定量测定霉菌毒素黄曲霉毒素B1。基于量子点的免疫分析比基于酶的免疫分析显示出更高的灵敏度。
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