Beloglazova Natalia V, Sobolev Aleksander M, Tessier Mickael D, Hens Zeger, Goryacheva Irina Yu, De Saeger Sarah
Ghent University, Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ottergemsesteenweg 460, 9000 Ghent, Belgium; Saratov State University, Chemistry Faculty, Department of General and Inorganic Chemistry, Astrakhanskaya 83, 410012 Saratov, Russia.
Ghent University, Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ottergemsesteenweg 460, 9000 Ghent, Belgium; Saratov State University, Chemistry Faculty, Department of General and Inorganic Chemistry, Astrakhanskaya 83, 410012 Saratov, Russia.
Methods. 2017 Mar 1;116:141-148. doi: 10.1016/j.ymeth.2017.01.004. Epub 2017 Jan 23.
A sensitive tool for simultaneous qualitative detection of two mycotoxins based on use of non-cadmium quantum dots (QDs) is presented for the first time. QDs have proven themselves as promising fluorescent labels for biolabeling and chemical analysis. With an increasing global tendency to regulate and limit the use of hazardous elements, indium phosphide (InP) QDs are highlighted as environmentally-friendly alternatives to the highly efficient and well-studied, but potentially toxic Cd- and Pb-based QDs. Here, we developed water-soluble InP QDs-based fluorescent nanostructures. They consisted of core/shell InP/ZnS QDs enrobed in a silica shell that allowed the water solubility (QD@SiO). Then we applied the QD@SiO as novel, silica shell-encapsulated fluorescent labels in immunoassays for rapid multiplexed screening. Two mycotoxins, zearalenone and deoxynivalenol, were simultaneously detected in maize and wheat, since the two QD@SiO labelled conjugates emit at two different, individually detectable wavelengths. The cutoff values for the simultaneous determination were 50 and 500μgkg for zearalenone and deoxynivalenol, respectively, in both maize and wheat. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was used to confirm the result.
首次提出了一种基于非镉量子点（QD）同时定性检测两种霉菌毒素的灵敏工具。量子点已被证明是用于生物标记和化学分析的有前途的荧光标记物。随着全球对危险元素使用进行监管和限制的趋势日益增强，磷化铟（InP）量子点作为高效且研究充分但可能有毒的镉基和铅基量子点的环保替代品而受到关注。在此，我们开发了基于水溶性InP量子点的荧光纳米结构。它们由包裹在二氧化硅壳中的核/壳InP/ZnS量子点组成，该二氧化硅壳赋予了水溶性（QD@SiO）。然后，我们将QD@SiO作为新型的、二氧化硅壳包裹的荧光标记物应用于免疫分析中，以进行快速多重筛选。在玉米和小麦中同时检测到了两种霉菌毒素，即玉米赤霉烯酮和脱氧雪腐镰刀菌烯醇，因为两种QD@SiO标记的缀合物在两个不同的、可单独检测的波长处发射荧光。在玉米和小麦中，同时测定玉米赤霉烯酮和脱氧雪腐镰刀菌烯醇的临界值分别为50和500μg/kg。采用液相色谱-串联质谱法（LC-MS/MS）对结果进行确认。
