Kobayashi K, Mitaka K, Asami T, Kitajima K, Takahashi K, Iwasaki S, Sasagawa I
Shigaku. 1989 Dec;77(4):1308-17.
The three-dimensional structure of the connective tissue core (CTC) of the four types of lingual papillae of the crab-eating macaque (Macaca fascicularis) was studied by scanning electron microscopy after removal of the epithelial cell layer by long-term treatment with hydrochloric acid at room temperature and compared with the results obtained from light microscopy. 1) Filiform papillae are densely distributed on the dorsal surface of the anterior two thirds of the tongue. Fungiform papillae are scattered among these filiform papillae, and are numerous at the anterior margin of the tongue. The connective tissue core of each filiform papilla looked like a lifting human hand with its palm towards the tongue tip. By using light microscopy, AF-positive elastic fibers were accumurated very densely just beneath the basal region of the anterior column epithelium of each filiform papilla. 2) CTC of fungiform papilla showed coralliform structure whose branches were ramified a few times. On the top of each branch there was a small depression where a taste bud was situated. 3) After removal of the epithelial cell layer of the foliate papilla, longitudinal grooves coinciding with the epithelial slits were observed. Some glandular ducts were seen protruding towards the exfoliated epithelium. 4) After removal of the covering epithelium of the vallate papilla, numerous small rod-shaped secondary CT cores appeared on the large primary CT core.
通过在室温下用盐酸长期处理去除上皮细胞层后，利用扫描电子显微镜研究了食蟹猕猴（Macaca fascicularis）四种舌乳头结缔组织核心（CTC）的三维结构，并与光学显微镜观察结果进行了比较。1）丝状乳头密集分布于舌前三分之二的背表面。菌状乳头散在于这些丝状乳头之间，在舌尖边缘较多。每个丝状乳头的结缔组织核心看起来像一只抬起的人手，手掌朝向舌尖。利用光学显微镜观察发现，在每个丝状乳头前柱上皮基部区域下方，AF阳性弹性纤维非常密集地聚集。2）菌状乳头的CTC呈珊瑚状结构，其分支有几次分支。每个分支顶端有一个小凹陷，味蕾位于此处。3）去除叶状乳头的上皮细胞层后，观察到与上皮裂隙一致的纵向沟。可见一些腺管向上皮脱落处突出。4）去除轮廓乳头的覆盖上皮后，在大的初级CT核心上出现许多小的棒状次级CT核心。
