Satoh M, Kameda A, Endo T
Shigaku. 1989 Dec;77(4):1327-42.
Resultant products and gel substance, formed with silicahydro gel method as well as with freeze-drying silicahydro gel method by use of tray, were compared and examined to materialize clinical application of gel method easily and the following results were obtained: 1. Evaluation of gel substance in control group gel (silicahydro gel) and in experimental group gel (gel prepared by the addition of deionized water to freeze-drying silicahydro gel) As the results of measuring the estimation of F- by ion chromatography and pH value, no clear-cut distinction between control group gel and experimental group gel was recognized, however, it was implied by naked-eye observations and through scanning electron microscope with the passage of time that experimental group gel had been inferior to control group gel. 2. Evaluation of resultant products in control group gel (resultant products formed with silicahydro gel method) and in experimental group gel (resultant products formed with freeze-drying silicahydro gel method) Naked-eye observations of reacting progress, the pH measurements, the calculation of Ca/P ratio, qualitative analyses by X-ray diffraction (reference; the measurements of crystallite size, lattice imperfection and lattice constant) and composition analyses by infrared absorption spectrum were carried out and as the results, resultant products in control group gel proved to be more favorable than those in experimental group gel. From the foregoing outcomes, it was suggested that fluorapatite of favorable crystallinity had been possible to be produced more with silicahydro gel method than with freeze-drying silicahydro gel method, and that further investigation inclusive of gel substance would be required in the event of clinically applying freeze-drying silicahydro gel method effectively.
对采用硅胶水凝胶法以及使用托盘的冷冻干燥硅胶水凝胶法形成的最终产物和凝胶物质进行了比较和检测，以实现凝胶法在临床应用中的简便性，并获得了以下结果：1. 对照组凝胶（硅胶水凝胶）和实验组凝胶（通过向冷冻干燥硅胶水凝胶中添加去离子水制备的凝胶）中凝胶物质的评估 通过离子色谱法测定氟离子含量以及测量pH值的结果表明，对照组凝胶和实验组凝胶之间没有明显区别，然而，通过肉眼观察和扫描电子显微镜随着时间的推移发现，实验组凝胶比对照组凝胶差。2. 对照组凝胶（采用硅胶水凝胶法形成的最终产物）和实验组凝胶（采用冷冻干燥硅胶水凝胶法形成的最终产物）中最终产物的评估 对反应进程进行肉眼观察、测量pH值、计算钙磷比、通过X射线衍射进行定性分析（参考：微晶尺寸、晶格缺陷和晶格常数的测量）以及通过红外吸收光谱进行成分分析，结果表明，对照组凝胶中的最终产物比实验组凝胶中的更有利。从上述结果来看，表明采用硅胶水凝胶法比冷冻干燥硅胶水凝胶法更有可能生产出结晶度良好的氟磷灰石，并且如果要有效地临床应用冷冻干燥硅胶水凝胶法，还需要对凝胶物质进行进一步研究。
