Nawaz Ahmad Ahsan, Nissly Ruth Helmus, Li Peng, Chen Yuchao, Guo Feng, Li Sixing, Shariff Yasir M, Qureshi Arooj Nawaz, Wang Lin, Huang Tony Jun
Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA, 16802, USA.
Ann Biomed Eng. 2014 Nov;42(11):2303-13. doi: 10.1007/s10439-014-1041-5. Epub 2014 Jun 4.
Cost-effective, high-performance diagnostic instruments are vital to providing the society with accessible, affordable, and high-quality healthcare. Here we present an integrated, "microfluidic drifting" based flow cytometry chip as a potential inexpensive, fast, and reliable diagnostic tool. It is capable of analyzing human blood for cell counting and diagnosis of diseases. Our device achieves a throughput of ~3754 events/s. Calibration with Flow-Check calibration beads indicated good congruency with a commercially available benchtop flow cytometer. Moreover, subjection to a stringent 8-peak rainbow calibration particle test demonstrated its ability to perform high-resolution immunological studies with separation resolution of 4.28 between the two dimmest fluorescent populations. Counting accuracy at different polystyrene bead concentrations showed strong correlation (r=0.9991) with hemocytometer results. Finally, reliable quantification of CD4+ cells in healthy human blood via staining with monoclonal antibodies was demonstrated. These results demonstrate the potential of our microfluidic flow cytometry chip as an inexpensive yet high-performance point-of-care device for mobile medicine.
具有成本效益的高性能诊断仪器对于为社会提供可及、可负担且高质量的医疗保健至关重要。在此，我们展示一种基于“微流体漂移”的集成流式细胞术芯片，作为一种潜在的廉价、快速且可靠的诊断工具。它能够分析人体血液以进行细胞计数和疾病诊断。我们的设备实现了约3754个事件/秒的通量。使用Flow-Check校准微珠进行校准表明，其与市售台式流式细胞仪具有良好的一致性。此外，经过严格的8峰彩虹校准颗粒测试，证明了它能够进行高分辨率免疫学研究，两个最暗荧光群体之间的分离分辨率为4.28。在不同聚苯乙烯微珠浓度下的计数准确性与血细胞计数器结果显示出强相关性（r = 0.9991）。最后，通过用单克隆抗体染色证明了对健康人血液中CD4 +细胞的可靠定量。这些结果证明了我们的微流体流式细胞术芯片作为一种用于移动医疗的廉价但高性能的即时检测设备的潜力。
