Entomology and Environment Program, Plant Production Technology Section, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Chalong Krung Rd, Ladkrabang, Bangkok, 10520, Thailand,
Parasitol Res. 2014 Aug;113(8):3015-22. doi: 10.1007/s00436-014-3964-z. Epub 2014 Jun 4.
The oviposition deterrent and ovicidal of seven herbal essential oils derived from Citrus sinensis, Cymbopogon citratus, Eucalyptus glubulus, Illicium verum, Lavandula angustifolia, Mentha piperita, and Zingiber cussumunar were assessed against the gravid female of housefly, Musca domestica L., under laboratory conditions and compared with commercial insecticide (10% w/v cypermethrin). They were assayed at three concentrations (1.0, 5.0, and 10.0%) where plastic cups containing 1 ml of desired oil concentration and cotton pad soaked with 10 ml of milk solution (10% w/v) were used as oviposition substrate. The 0.1 ml of deferent concentrations was dropped on ten housefly eggs, which were used for ovicidal activity. The number of eggs laid and the hatched larvae in each cup was recorded to evaluate the oviposition deterrent and ovicidal activities of the herbal essential oils. High concentration (10%) of herbal essential oils showed high percent effective repellency (ER). The 10% I. verum oil caused complete oviposition deterrence (100% ER, oviposition activity index (OAI) = -1.0), followed by Z. cussumunar, M. piperita, L. angustifolia, C. citratus, C. sinensis, and E. glubulus oils with 97.20, 88.55, 88.14, 87.93, 76.68, and 57.00% ER, respectively. As the concentration of herbal essential oils increased from 1.0, 5.0, and up to 10.0% concentration, the hatching rate decreased. Ten percent I. verum oil gave the maximum inhibiting rate at 97.3% (LC50 value of 6.85%); in addition, the other herbal essential oils showed the minimum inhibiting rate of 3.3-22.7%. On the other hand, cypermethrin 10% w/v showed complete oviposition deterrence (100% ER, OAI = -1.0) and ovicidal activity (100% inhibiting rate). Our data showed that I. verum oil have high potential of oviposition deterrence and ovicide housefly control.
七种植物精油（来源于甜橙、柠檬草、蓝桉、八角、薰衣草、胡椒薄荷和生姜）的产卵驱避和杀卵作用在实验室条件下对家蝇（Musca domestica L.）的孕雌蝇进行了评估，并与商业杀虫剂（10% w/v 氯菊酯）进行了比较。它们在三个浓度（1.0、5.0 和 10.0%）下进行了检测，其中使用塑料杯，每个杯内盛有 1 毫升所需油浓度的液体和浸有 10 毫升牛奶溶液（10% w/v）的棉花垫作为产卵基质。将不同浓度的 0.1 毫升液滴滴在 10 只家蝇卵上，用于评估杀卵活性。记录每个杯子中产卵的数量和孵化的幼虫数量，以评估植物精油的产卵驱避和杀卵活性。高浓度（10%）的植物精油表现出高的有效驱避率（ER）。10%的八角精油导致完全的产卵驱避（100% ER，产卵活动指数（OAI）=-1.0），其次是生姜、胡椒薄荷、薰衣草、柠檬草、甜橙和蓝桉精油，其 ER 分别为 97.20%、88.55%、88.14%、87.93%、76.68%和 57.00%。随着植物精油浓度从 1.0%、5.0%增加到 10.0%，孵化率降低。10%的八角精油的最大抑制率为 97.3%（LC50 值为 6.85%）；此外，其他植物精油的抑制率最低为 3.3-22.7%。另一方面，氯菊酯 10% w/v 表现出完全的产卵驱避（100% ER，OAI=-1.0）和杀卵活性（100%抑制率）。我们的数据表明，八角精油具有很高的家蝇产卵驱避和杀卵潜力。
