Jin Xuelong, Wang Feifei, Liu Xingju, Liang Bin, Chen Zequn, He Junfeng, Zhang Hong, Zhang Jianning
Department of Physiology, Tianjin Medical University, Tianjin, China.
Microcirculation. 2014 Nov;21(8):696-702. doi: 10.1111/micc.12150.
TBI causes localized cerebral ischemia that, in turn, is accompanied by both changes in BBB permeability and recruitment of CD34(+) cells to the injured tissue. However, it remains unknown whether CD34(+) cell recruitment is linked to BBB permeability. This study is a preliminary investigation into possible correlations between CD34(+) cell recruitment and BBB permeability following TBI in a rat model.
Male SD rats were subjected to mild fluid percussion injury. BBB permeability was assessed by measuring extrinsic EB dye extravasation and endogenous EBA expression at days 1, 3, 5, 7, and 12 post injury. The number of CD34(+) cells in the damaged tissue was analyzed by immunohistochemistry at each time point.
EB dye extravasation reached a peak at day 3 following TBI, while EBA expression displayed the reverse profile. Accumulation of CD34(+) cells in injured brain tissue was evident at five days post injury. It revealed a negative linear correlation between CD34(+) cell and BBB permeability.
The negative linear correlation between CD34(+) cell recruitment and BBB permeability following TBI provides a support for further study of CD34(+) cell transplantation for BBB repair after TBI.
创伤性脑损伤（TBI）会导致局部脑缺血，进而伴随着血脑屏障（BBB）通透性的改变以及CD34(+)细胞向损伤组织的募集。然而，CD34(+)细胞的募集是否与BBB通透性相关仍不清楚。本研究是对大鼠TBI模型中CD34(+)细胞募集与BBB通透性之间可能存在的相关性进行的初步调查。
雄性SD大鼠接受轻度液体冲击伤。在损伤后第1、3、5、7和12天，通过测量外源性伊文思蓝（EB）染料外渗和内源性伊文思蓝白蛋白（EBA）表达来评估BBB通透性。在每个时间点通过免疫组织化学分析受损组织中CD34(+)细胞的数量。
TBI后第3天EB染料外渗达到峰值，而EBA表达呈现相反的趋势。损伤后5天，CD34(+)细胞在受损脑组织中的积聚明显。结果显示CD34(+)细胞与BBB通透性之间呈负线性相关。
TBI后CD34(+)细胞募集与BBB通透性之间的负线性相关为进一步研究CD34(+)细胞移植修复TBI后的BBB提供了支持。
