Koch Jennifer L, Carey David W
Northern Research Station, US Forest Service;
Northern Research Station, US Forest Service.
J Vis Exp. 2014 May 27(87):51515. doi: 10.3791/51515.
Beech bark disease (BBD) results in high levels of initial mortality, leaving behind survivor trees that are greatly weakened and deformed. The disease is initiated by feeding activities of the invasive beech scale insect, Cryptococcus fagisuga, which creates entry points for infection by one of the Neonectria species of fungus. Without scale infestation, there is little opportunity for fungal infection. Using scale eggs to artificially infest healthy trees in heavily BBD impacted stands demonstrated that these trees were resistant to the scale insect portion of the disease complex(1). Here we present a protocol that we have developed, based on the artificial infestation technique by Houston(2), which can be used to screen for scale-resistant trees in the field and in smaller potted seedlings and grafts. The identification of scale-resistant trees is an important component of management of BBD through tree improvement programs and silvicultural manipulation.
山毛榉树皮病（BBD）会导致大量树木初期死亡，留下的存活树木则严重衰弱且变形。这种疾病是由入侵性的山毛榉球蚜Cryptococcus fagisuga的取食活动引发的，它为一种Neonectria属真菌的感染创造了入口。没有球蚜侵害，真菌感染的机会就很少。在受BBD严重影响的林分中，用球蚜卵人工感染健康树木，结果表明这些树木对该病害复合体中的球蚜部分具有抗性(1)。在此，我们介绍一种基于休斯顿的人工感染技术(2)开发的方案，该方案可用于在野外以及较小的盆栽幼苗和嫁接苗中筛选抗球蚜树木。识别抗球蚜树木是通过树木改良计划和造林操作来管理BBD的重要组成部分。
