State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), ‡College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University , Tianjin 300071, People's Republic of China.
ACS Appl Mater Interfaces. 2014 Jun 25;6(12):9815-21. doi: 10.1021/am502250r. Epub 2014 Jun 11.
We occasionally found that several self-assembling peptides containing D-amino acids would be preferentially enriched in cellular membranes at self-assembled stages while distributed evenly in the cytoplasma of cells at unassembled stages. Self-assembling peptides containing only Lamino acids distributed evenly in cytoplasma of cells at both self-assembled and unassembled stages. The self-assembling peptides containing D-amino acids could therefore be applied for engineering cell surface with peptides. More importantly, by integrating a protein binding peptide (a PDZ domain binding hexapeptide of WRESAI) with the self-assembling peptide containing D-amino acids, protein could also be introduced to the cell surface. This study not only provided a novel approach to engineer cell surface, but also highlighted the unusual properties and potential applications of self-assembling peptides containing D-amino acids in regenerative medicine, drug delivery, and tissue engineering.
我们偶尔会发现，几种含有 D-氨基酸的自组装肽在自组装阶段会优先富集在细胞膜中，而在未组装阶段则均匀分布在细胞质中。仅含有 L-氨基酸的自组装肽在自组装和未组装阶段均均匀分布在细胞质中。因此，含有 D-氨基酸的自组装肽可用于工程化细胞表面肽。更重要的是，通过将蛋白质结合肽（与 WRESAI 的 PDZ 结构域结合的六肽）与含有 D-氨基酸的自组装肽整合，也可以将蛋白质引入到细胞表面。这项研究不仅为工程化细胞表面提供了一种新方法，还突出了含有 D-氨基酸的自组装肽在再生医学、药物输送和组织工程中的特殊性质和潜在应用。
