小 IP₃R 簇模型中钙峰和钙脉冲的频率和相对发生率。
Frequency and relative prevalence of calcium blips and puffs in a model of small IP₃R clusters.
机构信息
Department of Physics and Institute of Theoretical Physics and Astrophysics, Xiamen University, Xiamen, China.
Institute of Physics, Humboldt-Universität zu Berlin, Germany.
出版信息
Biophys J. 2014 Jun 3;106(11):2353-63. doi: 10.1016/j.bpj.2014.04.027.
In this work, we model the local calcium release from clusters with a few inositol 1,4,5-trisphosphate receptor (IP3R) channels, focusing on the stochastic process in which an open channel either triggers other channels to open (as a puff) or fails to cause any channel to open (as a blip). We show that there are linear relations for the interevent interval (including blips and puffs) and the first event latency against the inverse cluster size. However, nonlinearity is found for the interpuff interval and the first puff latency against the inverse cluster size. Furthermore, the simulations indicate that the blip fraction among all release events and the blip frequency are increasing with larger basal [Ca(2+)], with blips in turn giving a growing contribution to basal [Ca(2+)]. This result suggests that blips are not just lapses to trigger puffs, but they may also possess a biological function to contribute to the initiation of calcium waves by a preceding increase of basal [Ca(2+)] in cells that have small IP3R clusters.
在这项工作中，我们通过几个肌醇 1,4,5-三磷酸受体（IP3R）通道对局部钙释放簇进行建模，重点研究了开放通道触发其他通道打开（作为一股爆发）或未能引起任何通道打开（作为一股微爆）的随机过程。我们表明，在簇的倒数与间隔时间（包括微爆和爆发）和第一事件潜伏期之间存在线性关系。然而，在间隔时间和第一个爆发潜伏期与簇的倒数之间发现了非线性关系。此外，模拟表明，在所有释放事件中，爆发之间的微爆分数和微爆频率随着基础 [Ca(2+)]的增加而增加，微爆反过来对基础 [Ca(2+)]的增加做出越来越大的贡献。这一结果表明，微爆不仅仅是触发爆发的失误，它们还可能通过增加基础 [Ca(2+)]来发挥生物功能，从而促进具有小 IP3R 簇的细胞中钙波的启动。
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