多发性骨髓瘤中肿瘤抗原的基因表达与CD4 + T细胞之间存在关联吗？
Is there any relationship between gene expression of tumor antigens and CD4+ T cells in multiple myeloma?
作者信息
Braga Walter Mt, da Silva Bruna R, Alves Veruska Lf, Bortoluzo Adriana B, Atanackovic Djordje, Colleoni Gisele Wb
机构信息
Rua Doutor Diogo de Faria, 824, 4° andar, CEP 04037 04003, São Paulo, Brazil.
出版信息
Immunotherapy. 2014;6(5):569-75. doi: 10.2217/imt.14.24.
AIM
The present study aimed at correlating the expression of cancer/testis antigens (CTAs) with the expression of genes related to tumor-infiltrating T cells.
MATERIALS & METHODS: MAGE-C1/CT-7, MAGEA3/6, NY-ESO-1, LAGE-1 and GAGE expression were evaluated in 46 bone marrow multiple myeloma (MM) aspirates by RT-PCR. Expression of FOXP3/CTLA4 and RORyt, as markers for Tregs and Th17 cells, respectively, was investigated by quantitative PCR.
RESULTS
MAGEC1/CT7 was expressed in 66% of MM samples. We did not find correlation between the presence of single CTA and expression of CTLA4 or RORyt neither expression of CD4(+) T-cell markers and the number of CTA simultaneously expressed in the tumor. However, we did observe a correlation between the percentage of plasma cells and the number of CTAs expressed in the patients' bone marrow.
CONCLUSION
Although CTAs and immunomodulatory CD4(+) T cells represent potential targets for immunotherapy in MM, we did not find association among expression of such genes in MM.
目的
本研究旨在关联癌/睾丸抗原（CTA）的表达与肿瘤浸润性T细胞相关基因的表达。
材料与方法
通过逆转录聚合酶链反应（RT-PCR）评估46例骨髓多发性骨髓瘤（MM）穿刺样本中黑色素瘤相关抗原C1/CT-7（MAGE-C1/CT-7）、黑色素瘤相关抗原A3/6（MAGEA3/6）、纽约食管鳞状细胞癌-1（NY-ESO-1）、淋巴细胞抗原6基因座A（LAGE-1）和G抗原基因家族（GAGE）的表达。分别通过定量聚合酶链反应研究叉头框蛋白P3（FOXP3）/细胞毒性T淋巴细胞相关抗原4（CTLA4）和维甲酸相关孤核受体γt（RORyt）作为调节性T细胞（Tregs）和辅助性T细胞17（Th17）细胞标志物的表达。
结果
66%的MM样本中表达MAGEC1/CT7。我们未发现单个CTA的存在与CTLA4或RORyt的表达之间存在相关性，也未发现CD4(+) T细胞标志物的表达与肿瘤中同时表达的CTA数量之间存在相关性。然而，我们确实观察到浆细胞百分比与患者骨髓中表达的CTA数量之间存在相关性。
结论
尽管CTA和免疫调节性CD4(+) T细胞是MM免疫治疗的潜在靶点，但我们未发现MM中此类基因表达之间存在关联。
Zotero 插件