Karn Santosh Kumar
Pak J Biol Sci. 2014 Feb 1;17(3):388-94. doi: 10.3923/pjbs.2014.388.394.
Chlorophenols contamination is serious concern to the environment due toxicity to all forms of life. Among all the chlorophenols, pentachlorophenol (PCP) is more detrimental to the environment. Pentachlorophenol used as pesticide, herbicide, antifungal agent and wood preservative which causes environmental pollution. In the present research a PCP degrading bacterium was isolated and characterized from industrial dump site. This isolate used PCP as its sole source of carbon and energy and was capable of degrading this compound, as indicated by stoichiometric release of chloride, ring cleavage activity and biomass formation. Based on morphological, biochemical and 16S rRNA gene sequence analysis this strain was identified as Enterobacter sp. SG1. Gas Chromatography (GC) analysis revealed that this strain was able to degrade PCP up to a concentration of 2 mM. This study showed that the removal efficiency of PCP by SG1 was found to be very effective and can be used in degradation of PCP contaminated site or waste in the environment.
由于对所有生命形式都具有毒性，氯酚污染已成为环境方面的严重问题。在所有氯酚中，五氯酚（PCP）对环境的危害更大。五氯酚用作杀虫剂、除草剂、抗真菌剂和木材防腐剂，会造成环境污染。在本研究中，从工业垃圾场分离并鉴定了一株能降解五氯酚的细菌。该分离株以五氯酚作为唯一的碳源和能源，并且能够降解该化合物，这通过氯离子的化学计量释放、环裂解活性和生物量形成得以证明。基于形态学、生化特性和16S rRNA基因序列分析，该菌株被鉴定为肠杆菌属SG1。气相色谱（GC）分析表明，该菌株能够降解浓度高达2 mM的五氯酚。本研究表明，SG1对五氯酚的去除效率非常高，可用于降解环境中受五氯酚污染的场地或废物。
