Department of Biotechnology, Thapar University, Patiala, India.
Biodegradation. 2011 Feb;22(1):63-9. doi: 10.1007/s10532-010-9376-6. Epub 2010 Jun 8.
A pentachlorophenol (PCP) degrading bacterium was isolated and characterized from sludge of pulp and paper mill. This isolate used PCP as its sole source of carbon and energy and was capable of degrading this compound, as indicated by stoichiometric release of chloride and biomass formation. Based on morphology, biochemical tests, and 16S rRNA gene sequence analysis this strain was identified as Kocuria sp. CL2. High Performance Liquid Chromatography (HPLC) analysis revealed that this strain was able to degrade PCP up to a concentration of 600 mg/l. This is first time we are reporting the degradation of PCP by the Kocuria species. This isolate was also able to remove 58.64% of PCP from the sludge within two weeks. This study showed that the removal efficiency of PCP by CL2 was found to be very effective and can be used in degradation of PCP containing pulp paper mill waste in the environment.
从制浆造纸厂的污泥中分离和鉴定出一种五氯苯酚（PCP）降解菌。该分离株将 PCP 用作其唯一的碳源和能源，并能够降解该化合物，这表明氯离子的化学计量释放和生物量的形成。根据形态、生化试验和 16S rRNA 基因序列分析，该菌株被鉴定为节杆菌属 CL2。高效液相色谱（HPLC）分析表明，该菌株能够将 PCP 降解至 600mg/L 的浓度。这是首次报道节杆菌属能够降解 PCP。该分离株还能够在两周内从污泥中去除 58.64%的 PCP。本研究表明，CL2 对 PCP 的去除效率非常有效，可用于降解环境中含 PCP 的制浆造纸厂废物。
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